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Literature summary for 1.3.3.5 extracted from

  • Han, X.; Zhao, M.; Lu, L.; Liu, Y.
    Purification, characterization and decolorization of bilirubin oxidase from Myrothecium verrucaria 3.2190 (2012), Fungal Biol., 116, 863-871.
    View publication on PubMed
Search for more literature for 1.3.3.5

Application

Application Comment Organism
additional information the purified bilirubin oxidase in Myrothecium verrucaria strain has potential application in dye effluent decolorization. Extracellular bilirubin oxidase decolorizes indigo carmine, biosorption and biodegradation of the dye is achieved with more than 98% decolorization efficiency after 7 days at 26°C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30°C to 50°C and pH 5.5-9.5 with dye concentrations of 50-200 mg/ml Albifimbria verrucaria

Inhibitors

Inhibitors Comment Organism Structure
DTT 62.3% inhibition at 1 mM Albifimbria verrucaria
K+
-
 Albifimbria verrucaria
L-cysteine 87% inhibition at 1 mM, no significant effect at 0.1 mM Albifimbria verrucaria
Li+
-
 Albifimbria verrucaria
additional information the enzyme is not affected by urea and EDTA Albifimbria verrucaria
Na+
-
 Albifimbria verrucaria
NaN3 76% inhibition at 10 mM Albifimbria verrucaria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 bilirubin pH 7.5, 25°C Albifimbria verrucaria

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Albifimbria verrucaria
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ highly activating, the enzyme contains four copper ions per functional unit Albifimbria verrucaria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 about, native PAGE Albifimbria verrucaria
68100
-
 1 * 68100, SDS-PAGE Albifimbria verrucaria

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 bilirubin + O2 Albifimbria verrucaria
-
 2 biliverdin + 2 H2O
-
 ?
2 bilirubin + O2 Albifimbria verrucaria 3.2190
-
 2 biliverdin + 2 H2O
-
 ?
additional information Albifimbria verrucaria extracellular bilirubin oxidase decolorizes indigo carmine, biosorption and biodegradation of the dye is achved with more than 98% decolorization efficiency after 7 days at 26°C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30°C to 50°C and pH 5.5-9.5 with dye concentrations of 50-200 mg/ml, overview ?
-
 ?
additional information Albifimbria verrucaria 3.2190 extracellular bilirubin oxidase decolorizes indigo carmine, biosorption and biodegradation of the dye is achved with more than 98% decolorization efficiency after 7 days at 26°C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30°C to 50°C and pH 5.5-9.5 with dye concentrations of 50-200 mg/ml, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Albifimbria verrucaria
-
-
-
Albifimbria verrucaria 3.2190
-
-
-

Purification (Commentary)

Purification (Comment) Organism
extracellular enzyme 3.92fold by ammonium sulfate fractionation and anion exchange chromatography, an additional step of gel filtration lowers the enzyme purity and amount Albifimbria verrucaria

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Albifimbria verrucaria
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
214.3
-
 purified enzyme after anion exchange chromatography step, pH 7.5, 25°C Albifimbria verrucaria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 bilirubin + O2
-
 Albifimbria verrucaria 2 biliverdin + 2 H2O
-
 ?
2 bilirubin + O2
-
 Albifimbria verrucaria 3.2190 2 biliverdin + 2 H2O
-
 ?
additional information extracellular bilirubin oxidase decolorizes indigo carmine, biosorption and biodegradation of the dye is achved with more than 98% decolorization efficiency after 7 days at 26°C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30°C to 50°C and pH 5.5-9.5 with dye concentrations of 50-200 mg/ml, overview Albifimbria verrucaria ?
-
 ?
additional information the enzyme is also active with the laccase substrate 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) Albifimbria verrucaria ?
-
 ?
additional information extracellular bilirubin oxidase decolorizes indigo carmine, biosorption and biodegradation of the dye is achved with more than 98% decolorization efficiency after 7 days at 26°C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30°C to 50°C and pH 5.5-9.5 with dye concentrations of 50-200 mg/ml, overview Albifimbria verrucaria 3.2190 ?
-
 ?
additional information the enzyme is also active with the laccase substrate 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) Albifimbria verrucaria 3.2190 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 68100, SDS-PAGE Albifimbria verrucaria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 substrate bilirubin Albifimbria verrucaria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 the enzyme activity is stable after incubation for 1 h at a temperature up to 40°C, thermal stability is decreased beyond 50?C Albifimbria verrucaria

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.19
-
 bilirubin pH 7.5, 25°C Albifimbria verrucaria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 substrate bilirubin Albifimbria verrucaria

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
104.3
-
 bilirubin pH 7.5, 25°C Albifimbria verrucaria