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Literature summary for 1.3.3.5 extracted from

  • Zhang, L.; Zhang, X.; Luo, Z.Y.
    Value of bilirubin oxidase and its mutants in the diagnosis of hyperbilirubinemia (2005), Di Yi Jun Yi Da Xue Xue Bao, 25, 1337-1340.
    View publication on PubMed
Search for more literature for 1.3.3.5

Application

Application Comment Organism
medicine mutants I402G and C457S are not appropriate for diagnostic purpose due to their much lower enzyme activities compared to the recombinant enzyme Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
C457S virtually no enzyme activity, Ru-incorporation conferrs higher enzyme activity Aspergillus oryzae
I402G low enzyme activity Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14.41
-
 bilirubin mutant I402G Aspergillus oryzae
24.22
-
 bilirubin recombinant enzyme Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 mutant C457S shows no activity Aspergillus oryzae
1.67
-
 mutant I402G Aspergillus oryzae
5.5
-
 mutant 1Ru-C457S Aspergillus oryzae
6.42
-
 mutant 2Ru-C457S Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bilirubin + O2
-
 Aspergillus oryzae biliverdin + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.115
-
 bilirubin mutant I402G Aspergillus oryzae
3.93
-
 bilirubin recombinant enzyme Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
6 7.6
-
 Aspergillus oryzae