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Literature summary for 1.3.3.5 extracted from

  • Cardenas-Vazquez, R.; Yokosuka, O.; Billing, B.H.
    Enzymic oxidation of unconjugated bilirubin by rat liver (1986), Biochem. J., 236, 625-633.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.3.5

Activating Compound

Activating Compound Comment Organism Structure
Salt maximum activity at a ionic strength of 60 mM, independent of salt type Rattus norvegicus
Triton X-100 0.01%, 25% increase in activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
albumin 0.008 mM, 50% inhibition Rattus norvegicus
Antimycin
-
 Rattus norvegicus
ATP
-
 Rattus norvegicus
bromosulphothalein 1 mM, 60% inhibition Rattus norvegicus
cysteine 1 mM, 40% inhibition Rattus norvegicus
Digitonin 0.1%, 50% inhibition Rattus norvegicus
dithiothreitol 1 mM, 60% inhibition Rattus norvegicus
KCN 0.03 mM, 70% inhibition Rattus norvegicus
mercaptoethanol 1 mM, 50% inhibition Rattus norvegicus
NADH 0.2 mM, 50% inhibition Rattus norvegicus
rotenone
-
 Rattus norvegicus
sodium taurocholate 0.5%, 50% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.136
-
 bilirubin
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion inner and outer membrane Rattus norvegicus 5739
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00157
-
 enzyme activity in liver mitochondria Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bilirubin + O2
-
 Rattus norvegicus biliverdin + H2O biliverdin + colourless diazo-negative compounds, including propentdyopents ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 70 10°C: about 36% of maximum, 70°C: about 10% of maximum Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 Tris-HCl buffer Rattus norvegicus
8.9 9.2 diethylethanolamine buffer Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
8 10 at pH 8.0 and 10.0: about 30% of maximum activity Rattus norvegicus