Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10b | Thermosynechococcus vestitus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen IX + 3 O2 | Thermosynechococcus vestitus | during heme and chlorophyll biosynthesis, the flavin-dependent protoporphyrinogen IX oxidase catalyzes the six-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. In the following step, iron is inserted into protoporphyrin IX by ferrochelatase. The two enzymes are organized in an enzyme complex, overview | protoporphyrin + 3 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
flavoprotein | - |
Thermosynechococcus vestitus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography | Thermosynechococcus vestitus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen IX + 3 O2 | - |
Thermosynechococcus vestitus | protoporphyrin + 3 H2O2 | - |
? | |
protoporphyrinogen IX + 3 O2 | during heme and chlorophyll biosynthesis, the flavin-dependent protoporphyrinogen IX oxidase catalyzes the six-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. In the following step, iron is inserted into protoporphyrin IX by ferrochelatase. The two enzymes are organized in an enzyme complex, overview | Thermosynechococcus vestitus | protoporphyrin + 3 H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | analysis of the enzyme in complex with ferrochelatase by mass spectrometry and immunohistochemical determination, overview | Thermosynechococcus vestitus |
Synonyms | Comment | Organism |
---|---|---|
PPO | - |
Thermosynechococcus vestitus |
protoporphyrinogen IX oxidase | - |
Thermosynechococcus vestitus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Thermosynechococcus vestitus |
General Information | Comment | Organism |
---|---|---|
metabolism | the flavin-dependent protoporphyrinogen IX oxidase catalyzes the six-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX in heme and chlorophyll biosynthesis. In the following step, iron is inserted into protoporphyrin IX by ferrochelatase. The two enzymes are organized in an enzyme complex, overview | Thermosynechococcus vestitus |