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Literature summary for 1.3.3.4 extracted from

  • Masoumi, A.; Heinemann, I.U.; Rohde, M.; Koch, M.; Jahn, M.; Jahn, D.
    Complex formation between protoporphyrinogen IX oxidase and ferrochelatase during haem biosynthesis in Thermosynechococcus elongatus (2008), Microbiology, 154, 3707-3714.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10b Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrinogen IX + 3 O2 Thermosynechococcus vestitus during heme and chlorophyll biosynthesis, the flavin-dependent protoporphyrinogen IX oxidase catalyzes the six-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. In the following step, iron is inserted into protoporphyrin IX by ferrochelatase. The two enzymes are organized in an enzyme complex, overview protoporphyrin + 3 H2O2
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Organism

Organism UniProt Comment Textmining
Thermosynechococcus vestitus
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Posttranslational Modification

Posttranslational Modification Comment Organism
flavoprotein
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Thermosynechococcus vestitus

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography Thermosynechococcus vestitus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrinogen IX + 3 O2
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Thermosynechococcus vestitus protoporphyrin + 3 H2O2
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protoporphyrinogen IX + 3 O2 during heme and chlorophyll biosynthesis, the flavin-dependent protoporphyrinogen IX oxidase catalyzes the six-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. In the following step, iron is inserted into protoporphyrin IX by ferrochelatase. The two enzymes are organized in an enzyme complex, overview Thermosynechococcus vestitus protoporphyrin + 3 H2O2
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?

Subunits

Subunits Comment Organism
More analysis of the enzyme in complex with ferrochelatase by mass spectrometry and immunohistochemical determination, overview Thermosynechococcus vestitus

Synonyms

Synonyms Comment Organism
PPO
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Thermosynechococcus vestitus
protoporphyrinogen IX oxidase
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Thermosynechococcus vestitus

Cofactor

Cofactor Comment Organism Structure
flavin
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Thermosynechococcus vestitus

General Information

General Information Comment Organism
metabolism the flavin-dependent protoporphyrinogen IX oxidase catalyzes the six-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX in heme and chlorophyll biosynthesis. In the following step, iron is inserted into protoporphyrin IX by ferrochelatase. The two enzymes are organized in an enzyme complex, overview Thermosynechococcus vestitus