Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)RIL cells | Homo sapiens |
expressed in Escherichia coli strain BL21(DE3)RIL | Homo sapiens |
into vector pET21d | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D400A | impaired catalytic ability, with 0.047% of wild-type total product efficiency | Homo sapiens |
D400A | the mutant produces no divinyl product, the Km value for coproporphyrinogen-III does not change significantly compared to the wild-type enzyme | Homo sapiens |
D400A | the mutation impairs the catalytic ability of CPO relative to the wild type enzyme, not able to form divinyl products, but the Km value for coproporphyrinogen-III does not change significantly compared to the wild-type enzyme | Homo sapiens |
R262A | impaired catalytic ability, with 0.00036% of wild-type total product efficiency | Homo sapiens |
R262A | poor catalyst for the production of a divinyl product with a catalytic efficiency less than 0.01% compared to wild-type, including a 15fold higher Km for coproporphyrinogen-III | Homo sapiens |
R262A | the mutant CPO is a poor catalyst for the production of a divinyl product, with a catalytic efficiency less than 0.01% compared to the wild type enzyme including a 15fold higher Km for coproporphyrinogen-III | Homo sapiens |
R401A | impaired catalytic ability, with 44% of wild-type total product efficiency | Homo sapiens |
R401A | the efficiency of divinyl product formation for mutant enzyme R401A is about 3% compared to wild type CPO, with a 3fold increase in the Km value for coproporphyrinogen-III | Homo sapiens |
R401A | the efficiency of divinyl product formation is about 3% compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
Harderoporphyrinogen | wild type enzyme | Homo sapiens | |
0.00034 | - |
Coproporphyrinogen-III | wild-type, production to intermediate divinyl | Homo sapiens | |
0.00056 | - |
mesoporphyrinogen-VI | wild type enzyme | Homo sapiens | |
0.00056 | - |
mesoporphyrinogen-VI | wild type enzyme, at 37°C | Homo sapiens | |
0.00056 | - |
mesoporphyrinogen-VI | wild-type, production to intermediate monovinyl | Homo sapiens | |
0.00078 | - |
Coproporphyrinogen-III | mutant D400A | Homo sapiens | |
0.00078 | - |
Coproporphyrinogen-III | mutant enzyme D400A | Homo sapiens | |
0.00078 | - |
Coproporphyrinogen-III | mutant enzyme D400A, at 37°C | Homo sapiens | |
0.00079 | - |
Coproporphyrinogen-III | wild-type | Homo sapiens | |
0.00079 | - |
Coproporphyrinogen-III | wild type enzyme | Homo sapiens | |
0.00079 | - |
Coproporphyrinogen-III | wild type enzyme, at 37°C | Homo sapiens | |
0.00096 | - |
Coproporphyrinogen-III | mutant R401A, production to intermediate divinyl | Homo sapiens | |
0.00096 | - |
Harderoporphyrinogen | mutant enzyme R401A | Homo sapiens | |
0.002 | - |
mesoporphyrinogen-VI | mutant R401A, production to intermediate monovinyl | Homo sapiens | |
0.002 | - |
mesoporphyrinogen-VI | mutant enzyme R401A | Homo sapiens | |
0.002 | - |
mesoporphyrinogen-VI | mutant enzyme R401A, at 37°C | Homo sapiens | |
0.0029 | - |
Coproporphyrinogen-III | mutant R401A | Homo sapiens | |
0.0029 | - |
Coproporphyrinogen-III | mutant enzyme R401A | Homo sapiens | |
0.0029 | - |
Coproporphyrinogen-III | mutant enzyme R401A, at 37°C | Homo sapiens | |
0.0051 | - |
Coproporphyrinogen-III | mutant R262A, production to intermediate divinyl | Homo sapiens | |
0.0051 | - |
Harderoporphyrinogen | mutant enzyme R262A | Homo sapiens | |
0.0087 | - |
Coproporphyrinogen-III | mutant R262A | Homo sapiens | |
0.0087 | - |
Coproporphyrinogen-III | mutant enzyme R262A | Homo sapiens | |
0.0087 | - |
Coproporphyrinogen-III | mutant enzyme R262A, at 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | P36551 | precursor | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose metal affinity resin chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
coproporphyrinogen III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O | CPO catalyzes two sequential oxidative decarboxylations of propionate moieties on coproporphyrinogen-III forming protoporphyrinogen-IX through the monovinyl intermediate harderoporphyrinogen | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-III + O2 + 2 H+ | - |
Homo sapiens | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
coproporphyrinogen-III + O2 + 2 H+ | Arg400, Arg262 and Arg401 are active amino acids critical for substrate binding and/or catalysis, Arg262 and 401 may coordinate carboxylate groups of coproporphyrinogen-III, while Asp400 may initiate deprotonation of substrate, resulting in oxidative decarboxylation | Homo sapiens | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
coproporphyrinogen-III + O2 + 2 H+ | - |
Homo sapiens | harderoporphyrinogen + CO2 + 2 H2O | - |
? | |
coproporphyrinogen-III + O2 + 2H+ | CPO catalyzes the conversion of coproporphyrinogen-III to protoporphyrinogen-IX, a divinyl product, via two sequential oxidative decarboxylations of the A and B ring propionates | Homo sapiens | protoporphyrinogen IX + 2 CO2 + 2 H2O | - |
? | |
harderoporphyrinogen + O2 | - |
Homo sapiens | protoporphyrinogen IX + CO2 + H2O2 | - |
? | |
harderoporphyrinogen + O2 + 2 H+ | - |
Homo sapiens | protoporphyrinogen-IX + CO2 + 2 H2O | - |
? | |
harderoporphyrinogen + O2 + H+ | - |
Homo sapiens | protoporphyrinogen IX + CO2 + H2O2 | - |
r | |
mesoporphyrinogen-VI + O2 + 2 H+ | - |
Homo sapiens | protoaetioporphyrin + 2 CO2 + 2 H2O | - |
? | |
mesoporphyrinogen-VI + O2 + 2 H+ | - |
Homo sapiens | protoaetioporphyrin + 2 CO2 + 2 H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CPO | - |
Homo sapiens |
CPO | CPO is the sixth enzyme in the heme biosynthetic pathway | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000123 | - |
Harderoporphyrinogen | mutant enzyme R262A | Homo sapiens | |
0.00002 | - |
Coproporphyrinogen-III | mutant D400A | Homo sapiens | |
0.00002 | - |
Coproporphyrinogen-III | mutant enzyme D400A | Homo sapiens | |
0.00002 | - |
Coproporphyrinogen-III | mutant enzyme D400A, at 37°C | Homo sapiens | |
0.0004167 | - |
Coproporphyrinogen-III | mutant R262A | Homo sapiens | |
0.00042 | - |
Coproporphyrinogen-III | mutant enzyme R262A, at 37°C | Homo sapiens | |
0.00167 | - |
Coproporphyrinogen-III | mutant R401A, production to intermediate divinyl | Homo sapiens | |
0.002 | - |
mesoporphyrinogen-VI | mutant R401A, production to intermediate monovinyl | Homo sapiens | |
0.002 | - |
mesoporphyrinogen-VI | mutant enzyme R401A | Homo sapiens | |
0.002 | - |
mesoporphyrinogen-VI | mutant enzyme R401A, at 37°C | Homo sapiens | |
0.0063 | - |
mesoporphyrinogen-VI | wild type enzyme, at 37°C | Homo sapiens | |
0.0063 | - |
mesoporphyrinogen-VI | wild-type, production to intermediate monovinyl | Homo sapiens | |
0.00633 | - |
mesoporphyrinogen-VI | wild type enzyme | Homo sapiens | |
0.017 | - |
Harderoporphyrinogen | mutant enzyme R401A | Homo sapiens | |
0.02 | - |
Coproporphyrinogen-III | mutant R401A | Homo sapiens | |
0.02 | - |
Coproporphyrinogen-III | mutant enzyme R401A | Homo sapiens | |
0.02 | - |
Coproporphyrinogen-III | mutant enzyme R401A, at 37°C | Homo sapiens | |
0.0267 | - |
Coproporphyrinogen-III | wild-type, production to intermediate divinyl | Homo sapiens | |
0.027 | - |
Harderoporphyrinogen | wild type enzyme | Homo sapiens | |
0.045 | - |
Coproporphyrinogen-III | wild-type | Homo sapiens | |
0.045 | - |
Coproporphyrinogen-III | wild type enzyme | Homo sapiens | |
0.045 | - |
Coproporphyrinogen-III | wild type enzyme, at 37°C | Homo sapiens | |
0.42 | - |
Coproporphyrinogen-III | mutant enzyme R262A | Homo sapiens |