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Literature summary for 1.3.3.3 extracted from

  • Yoshinaga, T.; Sano, S.
    Coproporphyrinogen oxidase. I. Purification, properties, and activation by phospholipids (1980), J. Biol. Chem., 255, 4722-4726.
    View publication on PubMed
Search for more literature for 1.3.3.3

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation Bos taurus
cardiolipin 2.2fold activation at 0.5 mg/ml Bos taurus
DTT slight activation Bos taurus
L-alpha-lysophosphatidylcholine 4.4fold activation at 0.5 mg/ml Bos taurus
L-alpha-lysophosphatidylethanolamine 3.4fold activation at 0.5 mg/ml Bos taurus
L-alpha-phosphatidyl-DL-glycerol 2.7fold activation at 0.5 mg/ml Bos taurus
L-alpha-phosphatidyl-L-serine 2.8fold activation at 0.5 mg/ml Bos taurus
L-alpha-phosphatidylcholine 3.8fold activation at 0.5 mg/ml Bos taurus
L-alpha-Phosphatidylethanolamine 3.9fold activation at 0.5 mg/ml Bos taurus
Lipids bovine liver lipids, 3.6fold activation at 1 mg/ml Bos taurus
phosphatidate 2.3fold activation at 0.5 mg/ml Bos taurus
Triton X-100 concentration-dependent activation up to 2.8fold Bos taurus
Tween 20 2.4 fold activation at 0.2%, v/v Bos taurus
Tween 40 2.2fold activation at 0.2%, v/v Bos taurus
Tween 60 2.3fold activation at 0.2%, v/v Bos taurus
Tween 80 2fold activation at 0.2%, v/v Bos taurus
Tween 85 1.8 fold activation at 0.2%, v/v Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
cetyltrimethylammonium chloride 86% inhibition at 0.05% Bos taurus
SDS 84% inhibition at 0.05%, w/v Bos taurus
sodium deoxycholate 71% inhibition at 0.05%, w/v Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018 0.025 Coproporphyrinogen-III presence of phospholipids Bos taurus
0.025
-
 Coproporphyrinogen-III crude extract Bos taurus
0.048
-
 Coproporphyrinogen-III purified enzyme Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal content Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71600
-
 1 * 71600, SDS-PAGE Bos taurus
74000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coproporphyrinogen-III + O2 + 2 H+ Bos taurus highly specific for oxygen as electron acceptor protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
4°C, 0.1 M Tris-HCl, pH 7.4, 50% saturation ammonium sulfate, 2 weeks Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen-III + O2 + 2 H+ highly specific for oxygen as electron acceptor Bos taurus protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 71600, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6.4 8.4 80% of the maximum activity within Bos taurus