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Literature summary for 1.3.2.3 extracted from

  • Leferink, N.G.; van Duijn, E.; Barendregt, A.; Heck, A.J.; van Berkel, W.J.
    Galactonolactone dehydrogenase requires a redox-sensitive thiol for optimal production of vitamin C (2009), Plant Physiol., 150, 596-605.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
into a pET-His6 vector for expression in Escherichia coli BL21DE3 cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C340A mutant, insensitive toward thiol oxidation, exhibits poor affinity for L-galactono-1,4-lactone Arabidopsis thaliana
C340S mutant, insensitive toward thiol oxidation, exhibits poor affinity for L-galactono-1,4-lactone Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) thiol-modifying agent Arabidopsis thaliana
H2O2 GALDH is inactivated by hydrogen peroxide due to selective oxidation of cysteine 340, located in the cap domain Arabidopsis thaliana
N-ethylmaleimide thiol-modifying agent Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
L-galactono-1,4-lactone wild-type enzyme Arabidopsis thaliana
5
-
L-galactono-1,4-lactone mutant C340S Arabidopsis thaliana
9
-
L-galactono-1,4-lactone mutant C340A Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactono-1,4-lactone + ferricytochrome c Arabidopsis thaliana
-
L-ascorbate + ferrocytochrome c + H+
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SU56
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactono-1,4-lactone + ferricytochrome c
-
Arabidopsis thaliana L-ascorbate + ferrocytochrome c + H+
-
?

Synonyms

Synonyms Comment Organism
galactonolactone dehydrogenase
-
Arabidopsis thaliana
GALDH
-
Arabidopsis thaliana
L-galactono-gamma-lactone dehydrogenase
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
59
-
L-galactono-1,4-lactone mutant C340S Arabidopsis thaliana
79
-
L-galactono-1,4-lactone mutant C340A Arabidopsis thaliana
134
-
L-galactono-1,4-lactone wild-type enzyme Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD
-
Arabidopsis thaliana