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Literature summary for 1.3.2.3 extracted from

  • Leferink, N.G.; Jose, M.D.; van den Berg, W.A.; van Berkel, W.J.
    Functional assignment of Glu386 and Arg388 in the active site of L-galactono-gamma-lactone dehydrogenase (2009), FEBS Lett., 583, 3199-3203.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Escherichia coli BL21DE3 cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
E386A mutant, catalytically far less efficient than wild-type GALDH, Glu386 is involved in productive substrate binding Arabidopsis thaliana
E386D mutant, catalytically far less efficient than wild-type GALDH, Glu386 is involved in productive substrate binding Arabidopsis thaliana
R388A inactive mutant, Arg388 is crucial for the stabilization of the anionic form of the reduced FAD cofactor Arabidopsis thaliana
R388K mutant, shows significant activity, Arg388 is crucial for the stabilization of the anionic form of the reduced FAD cofactor Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
L-galactono-1,4-lactone wild-type enzyme Arabidopsis thaliana
10.2
-
D-arabinono-1,4-lactone wild-type enzyme Arabidopsis thaliana
12.1
-
L-galactono-1,4-lactone mutant E386D Arabidopsis thaliana
12.4
-
L-gulono-1,4-lactone mutant E386A Arabidopsis thaliana
13.1
-
L-gulono-1,4-lactone wild-type enzyme Arabidopsis thaliana
17.9
-
L-galactono-1,4-lactone mutant E386A Arabidopsis thaliana
18.7
-
L-gulono-1,4-lactone mutant E386D Arabidopsis thaliana
107
-
D-arabinono-1,4-lactone mutant E386D Arabidopsis thaliana
113
-
L-galactono-1,4-lactone mutant R388A Arabidopsis thaliana
123
-
L-galactono-1,4-lactone mutant R388K Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial intermembrane space
-
Arabidopsis thaliana 5758
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactono-1,4-lactone + ferricytochrome c Arabidopsis thaliana
-
L-ascorbate + ferrocytochrome c + H+
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
via His-tag Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinono-1,4-lactone + ferrocytochrome c + H+
-
Arabidopsis thaliana ?
-
?
L-galactono-1,4-lactone + ferricytochrome c
-
Arabidopsis thaliana L-ascorbate + ferrocytochrome c + H+
-
?
L-gulono-1,4-lactone + ferricytochrome c
-
Arabidopsis thaliana L-ascorbate + ferrocytochrome c
-
?

Synonyms

Synonyms Comment Organism
GALDH
-
Arabidopsis thaliana
L-galactono-gamma-lactone dehydrogenase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
enzyme assay Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
L-gulono-1,4-lactone mutant E386A Arabidopsis thaliana
0.7
-
L-galactono-1,4-lactone mutant R388A Arabidopsis thaliana
1.2
-
D-arabinono-1,4-lactone mutant E386D Arabidopsis thaliana
1.4
-
L-galactono-1,4-lactone mutant E386A Arabidopsis thaliana
4
-
L-gulono-1,4-lactone wild-type enzyme Arabidopsis thaliana
4.7
-
L-galactono-1,4-lactone mutant E386D Arabidopsis thaliana
10.4
-
L-gulono-1,4-lactone mutant E386D Arabidopsis thaliana
48.5
-
L-galactono-1,4-lactone mutant R388K Arabidopsis thaliana
51
-
D-arabinono-1,4-lactone wild-type enzyme Arabidopsis thaliana
134
-
L-galactono-1,4-lactone wild-type enzyme Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
enzyme assay Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD
-
Arabidopsis thaliana