Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli BL21DE3 cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
E386A | mutant, catalytically far less efficient than wild-type GALDH, Glu386 is involved in productive substrate binding | Arabidopsis thaliana |
E386D | mutant, catalytically far less efficient than wild-type GALDH, Glu386 is involved in productive substrate binding | Arabidopsis thaliana |
R388A | inactive mutant, Arg388 is crucial for the stabilization of the anionic form of the reduced FAD cofactor | Arabidopsis thaliana |
R388K | mutant, shows significant activity, Arg388 is crucial for the stabilization of the anionic form of the reduced FAD cofactor | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
L-galactono-1,4-lactone | wild-type enzyme | Arabidopsis thaliana | |
10.2 | - |
D-arabinono-1,4-lactone | wild-type enzyme | Arabidopsis thaliana | |
12.1 | - |
L-galactono-1,4-lactone | mutant E386D | Arabidopsis thaliana | |
12.4 | - |
L-gulono-1,4-lactone | mutant E386A | Arabidopsis thaliana | |
13.1 | - |
L-gulono-1,4-lactone | wild-type enzyme | Arabidopsis thaliana | |
17.9 | - |
L-galactono-1,4-lactone | mutant E386A | Arabidopsis thaliana | |
18.7 | - |
L-gulono-1,4-lactone | mutant E386D | Arabidopsis thaliana | |
107 | - |
D-arabinono-1,4-lactone | mutant E386D | Arabidopsis thaliana | |
113 | - |
L-galactono-1,4-lactone | mutant R388A | Arabidopsis thaliana | |
123 | - |
L-galactono-1,4-lactone | mutant R388K | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial intermembrane space | - |
Arabidopsis thaliana | 5758 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + ferricytochrome c | Arabidopsis thaliana | - |
L-ascorbate + ferrocytochrome c + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
via His-tag | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinono-1,4-lactone + ferrocytochrome c + H+ | - |
Arabidopsis thaliana | ? | - |
? | |
L-galactono-1,4-lactone + ferricytochrome c | - |
Arabidopsis thaliana | L-ascorbate + ferrocytochrome c + H+ | - |
? | |
L-gulono-1,4-lactone + ferricytochrome c | - |
Arabidopsis thaliana | L-ascorbate + ferrocytochrome c | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GALDH | - |
Arabidopsis thaliana |
L-galactono-gamma-lactone dehydrogenase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme assay | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
L-gulono-1,4-lactone | mutant E386A | Arabidopsis thaliana | |
0.7 | - |
L-galactono-1,4-lactone | mutant R388A | Arabidopsis thaliana | |
1.2 | - |
D-arabinono-1,4-lactone | mutant E386D | Arabidopsis thaliana | |
1.4 | - |
L-galactono-1,4-lactone | mutant E386A | Arabidopsis thaliana | |
4 | - |
L-gulono-1,4-lactone | wild-type enzyme | Arabidopsis thaliana | |
4.7 | - |
L-galactono-1,4-lactone | mutant E386D | Arabidopsis thaliana | |
10.4 | - |
L-gulono-1,4-lactone | mutant E386D | Arabidopsis thaliana | |
48.5 | - |
L-galactono-1,4-lactone | mutant R388K | Arabidopsis thaliana | |
51 | - |
D-arabinono-1,4-lactone | wild-type enzyme | Arabidopsis thaliana | |
134 | - |
L-galactono-1,4-lactone | wild-type enzyme | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
enzyme assay | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana |