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Literature summary for 1.3.1.B7 extracted from

  • Rajavel, M.; Perinbam, K.; Gopal, B.
    Structural insights into the role of Bacillus subtilis YwfH (BacG) in tetrahydrotyrosine synthesis (2013), Acta Crystallogr. Sect. D, 69, 324-332.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of YwfH is determined in three conformational states. These represent apo YwfH, the YwfH/NADPH complex and an apo-like form Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
K113A kcat/Km is 4.9fold lower than the wild-type value Bacillus subtilis
N158A kcat/Km is 4.6fold lower than the wild-type value Bacillus subtilis
S155A kcat/Km is 5.5fold lower than the wild-type value Bacillus subtilis
S250A kcat/Km is 2.2fold lower than the wild-type value Bacillus subtilis
Y117A kcat/Km is 5.8fold lower than the wild-type value Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.178
-
NADPH pH and temperature not specified in the publication, wild-type enzyme Bacillus subtilis
0.365
-
NADPH pH and temperature not specified in the publication, mutant enzyme K113A Bacillus subtilis
323
-
NADPH pH and temperature not specified in the publication, mutant enzyme S250A Bacillus subtilis
345
-
NADPH pH and temperature not specified in the publication, mutant enzyme N158A Bacillus subtilis
528
-
NADPH pH and temperature not specified in the publication, mutant enzyme Y117A Bacillus subtilis
614
-
NADPH pH and temperature not specified in the publication, mutant enzyme S155A Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
2 * 31000 Bacillus subtilis
61500
-
analytical size-exclusion chromatography Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3E)-3-[(4S)-4-hydroxycyclohex-2-en-1-yl]-2-oxopropanoate + NADPH + H+
-
Bacillus subtilis 3-[(1R,4S)-4-hydroxycyclohex-2-en-1-yl]-2-oxopropanoate + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 31000 Bacillus subtilis

Synonyms

Synonyms Comment Organism
BacG
-
Bacillus subtilis
YwfH
-
Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0344
-
NADPH pH and temperature not specified in the publication, wild-type enzyme Bacillus subtilis
0.143
-
NADPH pH and temperature not specified in the publication, mutant enzyme N158A Bacillus subtilis
0.146
-
NADPH pH and temperature not specified in the publication, mutant enzyme K113A Bacillus subtilis
0.175
-
NADPH pH and temperature not specified in the publication, mutant enzyme Y117A Bacillus subtilis
0.216
-
NADPH pH and temperature not specified in the publication, mutant enzyme S155A Bacillus subtilis
0.286
-
NADPH pH and temperature not specified in the publication, mutant enzyme S250A Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.033
-
NADPH pH and temperature not specified in the publication, mutant enzyme Y117A Bacillus subtilis
0.035
-
NADPH pH and temperature not specified in the publication, mutant enzyme S155A Bacillus subtilis
0.04
-
NADPH pH and temperature not specified in the publication, mutant enzyme K113A Bacillus subtilis
0.042
-
NADPH pH and temperature not specified in the publication, mutant enzyme N158A Bacillus subtilis
0.089
-
NADPH pH and temperature not specified in the publication, mutant enzyme S250A Bacillus subtilis
0.193
-
NADPH pH and temperature not specified in the publication, wild-type enzyme Bacillus subtilis