Literature summary for 1.3.1.98 extracted from

Shapiro, A.B.; Livchak, S.; Gao, N.; Whiteaker, J.; Thresher, J.; Jahic, H.; Huang, J.; Gu, R.F.
A homogeneous, high-throughput-compatible, fluorescence intensity-based assay for UDP-N-acetylenolpyruvylglucosamine reductase (MurB) with nanomolar product detection (2012), J. Biomol. Screen., 17, 327-338.

Search for more literature for 1.3.1.98

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli DH5alpha cells	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not influenced by DMSO	Escherichia coli
NADP+	about 78% inhibition at 0.06 mM	Escherichia coli
thio-NADP+	about 80% inhibition at 0.06 mM	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.001	-	UDP-N-acetylenolpyruvylglucosamine	10 mM Mg2+, 50 mM Tris-HCl, at pH 8.0, temperature not specified in the publication	Escherichia coli
0.0016	-	NADPH	in 10 mM PIPES-NaOH (pH 7.0), 3 mM KCl, 2 mM dithiothreitol, 1 mM MgCl, temperature not specified in the publication	Escherichia coli
0.0039	-	UDP-N-acetylenolpyruvylglucosamine	10 mM Mg2+, 50 mM Tris-HCl, at pH 8.0 temperature not specified in the publication	Escherichia coli
0.0046	-	NADPH	10 mM Mg2+, 50 mM Tris-HCl, at pH 8.0, temperature not specified in the publication	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Escherichia coli	5737	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	K+ activates at 3 mM	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37800	-	x * 37800	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Superdex 200 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetylenolpyruvylglucosamine + NADPH + H+	-	Escherichia coli	UDP-N-acetylmuramate + NADP+	-	?

Subunits

Subunits	Comment	Organism
?	x * 37800	Escherichia coli

Synonyms

Synonyms	Comment	Organism
EC 1.1.1.158	formerly	Escherichia coli
MurB	-	Escherichia coli
UDP-N-acetylenolpyruvylglucosamine reductase	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Escherichia coli

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Release 2023.1 (January 2023)