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Literature summary for 1.3.1.92 extracted from

  • Zhang, Y.; Teoh, K.H.; Reed, D.W.; Maes, L.; Goossens, A.; Olson, D.J.; Ross, A.R.; Covello, P.S.
    The molecular cloning of artemisinic aldehyde DELTA11(13) reductase and its role in glandular trichome-dependent biosynthesis of artemisinin in Artemisia annua (2008), J. Biol. Chem., 283, 21501-21508.
    View publication on PubMed
Search for more literature for 1.3.1.92

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Artemisia annua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 artemisinic aldehyde 30°C, pH 7.5 Artemisia annua
0.095
-
 NADPH 30°C, pH 7.5 Artemisia annua
0.65
-
 (+)-carvone 30°C, pH 7.5 Artemisia annua
0.77
-
 NADH 30°C, pH 7.5 Artemisia annua
0.79
-
 2-cyclohexen-1-one 30°C, pH 7.5 Artemisia annua

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Artemisia annua 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 gel filtration Artemisia annua

Organism

Organism UniProt Comment Textmining
Artemisia annua C5H429
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Artemisia annua

Source Tissue

Source Tissue Comment Organism Textmining
flower flower bud, moderate expression Artemisia annua
-
leaf moderate expression Artemisia annua
-
trichome glandular trichome, high expression Artemisia annua
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-carvone + NADH + H+
-
 Artemisia annua (1R,4S)-isodihydrocarvone + NAD+ plus about 20% of (1S,4S)-dihydrocarvone ?
2-cyclohexen-1-one + NADH + H+
-
 Artemisia annua cyclohexanone + NAD+
-
 ?
2E-nonenal + NADH + H+ about 12% of the activity with artemisinic aldehyde Artemisia annua nonanal + NAD+
-
 ?
artemisinic aldehyde + NADH + H+
-
 Artemisia annua (11R)-dihydroartemisinic aldehyde + NAD+
-
 ?
artemisinic aldehyde + NADPH + H+
-
 Artemisia annua dihydroartemisinic aldehyde + NADP+
-
 ?
additional information no substrate: arteannuin B, artemisinic acid, artemisinic alcohol, artemisitene, coniferyl aldehyde, (2E)-nonenal, alpha-pinene, (+)-pulegone, and sabinone Artemisia annua ?
-
 ?

Synonyms

Synonyms Comment Organism
Dbr2
-
 Artemisia annua

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.86
-
 (+)-carvone 30°C, pH 7.5 Artemisia annua
1.3
-
 NADH 30°C, pH 7.5 Artemisia annua
1.8
-
 2-cyclohexen-1-one 30°C, pH 7.5 Artemisia annua
2.5
-
 NADPH 30°C, pH 7.5 Artemisia annua
2.6
-
 artemisinic aldehyde 30°C, pH 7.5 Artemisia annua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Artemisia annua

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
 complete inhibition Artemisia annua
9
-
 about 25% of maximum activity Artemisia annua

Cofactor

Cofactor Comment Organism Structure
NADH NADPH is preferred over NADH Artemisia annua
NADPH NADPH is preferred over NADH Artemisia annua

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 (+)-carvone 30°C, pH 7.5 Artemisia annua
1.7
-
 NADH 30°C, pH 7.5 Artemisia annua
2.3
-
 2-cyclohexen-1-one 30°C, pH 7.5 Artemisia annua
26
-
 NADPH 30°C, pH 7.5 Artemisia annua
140
-
 artemisinic aldehyde 30°C, pH 7.5 Artemisia annua