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Literature summary for 1.3.1.9 extracted from

  • Shaw, D.J.; Robb, K.; Vetter, B.V.; Tong, M.; Molle, V.; Hunt, N.T.; Hoskisson, P.A.
    Disruption of key NADH-binding pocket residues of the Mycobacterium tuberculosis InhA affects DD-CoA binding ability (2017), Sci. Rep., 7, 4714 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.9

Protein Variants

Protein Variants Comment Organism
M155A vmax for NADH is similar to that of wild-type, the M155A Vmax for trans-2-dodecenoyl-CoA is around 64% of the wild-type rate Mycobacterium tuberculosis
P193A mutation inactivates the ability of InhA to turn over either NADH or trans-2-dodecenoyl-CoA Mycobacterium tuberculosis
S94A similar activity compared to wild-type but a reduced NADH affinity, the affinity of S94A for is also decreased Mycobacterium tuberculosis
W222A reduction in overall enoyl reductase activity, mutant shows an increased affinity for NADH Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 NADH mutant P193A, pH 7.5, 37°C Mycobacterium tuberculosis
0.026
-
 trans-2-dodecenoyl-CoA mutant M155A, pH 7.5, 37°C Mycobacterium tuberculosis
0.053
-
 trans-2-dodecenoyl-CoA mutant P193A, pH 7.5, 37°C Mycobacterium tuberculosis
0.065
-
 NADH mutant M155A, pH 7.5, 37°C Mycobacterium tuberculosis
0.075
-
 trans-2-dodecenoyl-CoA wild-type, pH 7.5, 37°C Mycobacterium tuberculosis
0.083
-
 NADH mutant W222A, pH 7.5, 37°C Mycobacterium tuberculosis
0.098
-
 NADH wild-type, pH 7.5, 37°C Mycobacterium tuberculosis
0.106
-
 trans-2-dodecenoyl-CoA mutant S94A, pH 7.5, 37°C Mycobacterium tuberculosis
0.145
-
 trans-2-dodecenoyl-CoA mutant W222A, pH 7.5, 37°C Mycobacterium tuberculosis
0.211
-
 NADH mutant S94A, pH 7.5, 37°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-2-dodecenoyl-CoA + NADH + H+
-
 Mycobacterium tuberculosis dodecanoyl-CoA + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
InhA
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 NADH mutant P193A, pH 7.5, 37°C Mycobacterium tuberculosis
3.8
-
 trans-2-dodecenoyl-CoA mutant P193A, pH 7.5, 37°C Mycobacterium tuberculosis
7.4
-
 trans-2-dodecenoyl-CoA mutant M155A, pH 7.5, 37°C Mycobacterium tuberculosis
10.3
-
 NADH mutant W222A, pH 7.5, 37°C Mycobacterium tuberculosis
11.6
-
 trans-2-dodecenoyl-CoA wild-type, pH 7.5, 37°C Mycobacterium tuberculosis
13.2
-
 trans-2-dodecenoyl-CoA mutant S94A, pH 7.5, 37°C Mycobacterium tuberculosis
17
-
 NADH wild-type, pH 7.5, 37°C Mycobacterium tuberculosis
17.6
-
 trans-2-dodecenoyl-CoA mutant W222A, pH 7.5, 37°C Mycobacterium tuberculosis
17.9
-
 NADH mutant M155A, pH 7.5, 37°C Mycobacterium tuberculosis
29.1
-
 NADH mutant S94A, pH 7.5, 37°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.3
-
 trans-2-dodecenoyl-CoA mutant P193A, pH 7.5, 37°C Mycobacterium tuberculosis
20
-
 NADH mutant P193A, pH 7.5, 37°C Mycobacterium tuberculosis
122
-
 trans-2-dodecenoyl-CoA mutant W222A, pH 7.5, 37°C Mycobacterium tuberculosis
123
-
 trans-2-dodecenoyl-CoA mutant S94A, pH 7.5, 37°C Mycobacterium tuberculosis
125
-
 NADH mutant W222A, pH 7.5, 37°C Mycobacterium tuberculosis
142
-
 NADH mutant S94A, pH 7.5, 37°C Mycobacterium tuberculosis
155
-
 trans-2-dodecenoyl-CoA wild-type, pH 7.5, 37°C Mycobacterium tuberculosis
173
-
 NADH wild-type, pH 7.5, 37°C Mycobacterium tuberculosis
277
-
 NADH mutant M155A, pH 7.5, 37°C Mycobacterium tuberculosis
283
-
 trans-2-dodecenoyl-CoA mutant M155A, pH 7.5, 37°C Mycobacterium tuberculosis