Literature summary for 1.3.1.9 extracted from

Kim, S.H.; Khan, R.; Choi, K.; Lee, S.W.; Rhee, S.
A triclosan-resistance protein from the soil metagenome is a novel enoyl-acyl carrier protein reductase Structure-guided functional analysis (2020), FEBS J., 287, 4710-4728 .

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Protein Variants

Protein Variants	Comment	Organism
K266A	strain carrying the mutation is not viable at nonpermissive temperature. 1% of wild-type activity	uncultured bacterium
Y260F	strain carrying the mutation is not viable at nonpermissive temperature. 5.7% of wild-type activity	uncultured bacterium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.043	-	NADH	pH 5.5, 30°C	uncultured bacterium
1.26	-	crotonoyl-CoA	pH 5.5, 30°C	uncultured bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	A0A1C9HA64	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
crotonoyl-CoA + NADH + H+	-	uncultured bacterium	butanoyl-CoA + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
FabMG	-	uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
38	-	NADH	pH 5.5, 30°C	uncultured bacterium
100	-	crotonoyl-CoA	pH 5.5, 30°C	uncultured bacterium

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	uncultured bacterium

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Release 2023.1 (January 2023)