Crystallization (Comment) | Organism |
---|---|
to 1.6 A resolution, space group P212121. The model consists of one monomer in the asymmetric unit which is composed of 13 alpha-helices and 11 beta-strands, representing a canonical Rossmann fold architecture. In addition to the conserved residues Y236 and K245 in the Y-X8-K motif, Y53, D111 and Y226 are key residues implicated in the reductase activity, and F113 and T276 are also important for enzyme function | Xanthomonas oryzae |
Protein Variants | Comment | Organism |
---|---|---|
D111A | inactive | Xanthomonas oryzae |
F113A | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
K245A | inactive | Xanthomonas oryzae |
K245R | inactive | Xanthomonas oryzae |
S50A | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
T276A | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
V246A | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
Y226F | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
Y236A | inactive | Xanthomonas oryzae |
Y236F | inactive | Xanthomonas oryzae |
Y53A | partly restores fatty acid synthesis in an Escherichia coli fabI mutant strain | Xanthomonas oryzae |
Y53F | restores fatty acid synthesis in an Escherichia coli fabI mutant strain to wild-type level | Xanthomonas oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0187 | - |
NADH | pH 7.5, 25°C | Xanthomonas oryzae | |
0.293 | - |
crotonyl-CoA | pH 7.5, 25°C | Xanthomonas oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas oryzae | Q2P9J6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NADH + H+ | - |
Xanthomonas oryzae | butyryl-CoA + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FabV | - |
Xanthomonas oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.25 | - |
crotonyl-CoA | pH 7.5, 25°C | Xanthomonas oryzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Xanthomonas oryzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
75 | - |
crotonyl-CoA | pH 7.5, 25°C | Xanthomonas oryzae |