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Literature summary for 1.3.1.9 extracted from

  • Dayan, F.E.; Ferreira, D.; Wang, Y.H.; Khan, I.A.; McInroy, J.A.; Pan, Z.
    A pathogenic fungi diphenyl ether phytotoxin targets plant enoyl (acyl carrier protein) reductase (2008), Plant Physiol., 147, 1062-1071.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
2-(3-hydroxy-5-methylphenoxy)-5-methoxy-3-methyl phenol cyperin Arabidopsis thaliana
5-chloro-2-(2,4-dichlorophenoxy)-phenol Triclosan Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.288
-
crotonyl-CoA in 10 mM sodium phosphate, pH 7.2, at 25°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
variant Columbia [Col-0]
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + NADH + H+
-
Arabidopsis thaliana butyryl-CoA + NAD+
-
?

Synonyms

Synonyms Comment Organism
enoyl (acyl carrier protein) reductase
-
Arabidopsis thaliana
ENR
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADH
-
Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0136
-
2-(3-hydroxy-5-methylphenoxy)-5-methoxy-3-methyl phenol in 10 mM sodium phosphate, pH 7.2, at 25°C Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000046
-
in 10 mM sodium phosphate, pH 7.2, at 25°C Arabidopsis thaliana 2-(3-hydroxy-5-methylphenoxy)-5-methoxy-3-methyl phenol
0.089
-
in 10 mM sodium phosphate, pH 7.2, at 25°C Arabidopsis thaliana 5-chloro-2-(2,4-dichlorophenoxy)-phenol