Literature summary for 1.3.1.9 extracted from

Ward, W.H.J.; Holdgate, G.A.; Rowsell, S.; McLean, E.G.; Pauptit, R.A.; Clayton, E.; Nichols, W.W.; Colls, J.G.; Minshull, C.A.; Jude, D.A.; Mistry, A.; Timms, D.; Camble, R.; Hales, N.J.; Britton, C.J.; Taylor, I.W.F.
Kinetic and structural characteristics of the inhibition of enoyl (acyl carrier protein) reductase by triclosan (1999), Biochemistry, 38, 12514-12525.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
complexed with NAD+ and triclosan, hanging drop vapor diffusion method	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
[5-chloro-2-(2,4-dichlorophenoxy)phenol]	i.e. triclosan, concentration required for 50% inhibition approximates to 50% of the enzyme concentration, competitive with respect to NADH, uncompetitive with respect to NAD+, inhibition is reversible and promoted by NAD+	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4	-	crotonyl-CoA	-	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AEK4	-	-

Purification (Commentary)

Purification (Comment)	Organism
95% purity	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00278	-	crotonyl CoA	-	Escherichia coli

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Release 2023.1 (January 2023)