Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Clostridioides difficile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Ferredoxin | pH 7.5, temperature not specified in the publication | Clostridioides difficile | |
0.0025 | - |
crotonyl-CoA | pH 7.5, temperature not specified in the publication | Clostridioides difficile | |
0.01 | - |
butyryl-CoA | pH 7.5, temperature not specified in the publication | Clostridioides difficile | |
0.145 | - |
NADH | pH 7.5, temperature not specified in the publication | Clostridioides difficile |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
- |
Clostridioides difficile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NADH + ferredoxin | - |
Clostridioides difficile | butyryl-CoA + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bcd2 | - |
Clostridioides difficile |
butyryl-CoA dehydrogenase complex | - |
Clostridioides difficile |
CD1054 | - |
Clostridioides difficile |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | butyryl-CoA | pH 7.5, temperature not specified in the publication | Clostridioides difficile | |
3 | 6 | NADH | pH 7.5, temperature not specified in the publication | Clostridioides difficile | |
19 | - |
crotonyl-CoA | pH 7.5, temperature not specified in the publication | Clostridioides difficile | |
37 | - |
Ferredoxin | pH 7.5, temperature not specified in the publication | Clostridioides difficile |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Clostridioides difficile |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | in the absence of ferredoxin, a remarkable stimulation of crotonyl-CoA reduction by FAD is observed | Clostridioides difficile | |
Ferredoxin | butyryl-CoA dehydrogenase from C. difficile belongs to the subfamily of bifurcating enzymes capable of coupling the exergonic reduction of crotonyl-CoA by NAD Hwith the endergonic reduction of ferredoxin by NADH | Clostridioides difficile | |
NADH | - |
Clostridioides difficile |
General Information | Comment | Organism |
---|---|---|
metabolism | butyryl-CoA dehydrogenase from C. difficile belongs to the subfamily of bifurcating enzymes capable of coupling the exergonic reduction of crotonyl-CoA by NAD Hwith the endergonic reduction of ferredoxin by NADH | Clostridioides difficile |
metabolism | the genes necessary for butyrate formation from the genome of Clostridium difficile are expressed in Escherichia coli. The individual genes are assembled in a single plasmid vector into an artificial operon , which allows functional coexpression of the required genes and confers butyrate-forming capability to the host | Clostridioides difficile |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
250 | - |
NADH | pH 7.5, temperature not specified in the publication | Clostridioides difficile | |
2800 | - |
butyryl-CoA | pH 7.5, temperature not specified in the publication | Clostridioides difficile | |
7600 | - |
crotonyl-CoA | pH 7.5, temperature not specified in the publication | Clostridioides difficile | |
18500 | - |
Ferredoxin | pH 7.5, temperature not specified in the publication | Clostridioides difficile |