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Literature summary for 1.3.1.78 extracted from

  • Legrand, P.; Dumas, R.; Seux, M.; Rippert, P.; Ravelli, R.; Ferrer, J.L.; Matringe, M.
    Biochemical characterization and crystal structure of Synechocystis arogenate dehydrogenase provide insights into catalytic reaction (2006), Structure, 14, 767-776.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop method, structure of TyrAsy in complex with NADP+ is refined to 1.6 A. The asymmetric unit contains two TyrAsy homodimers, with each monomer consisting of a nucleotide binding N-terminal domain and a particularly unique alpha-helical C-terminal dimerization domain Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information almost completely insensitive to feedback inhibition by tyrosine. Phenylalanine and tryptophan show no effect at concentrations of up to 1 mM Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
NADP+
-
Synechocystis sp.
0.093
-
L-arogenate
-
Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
2 * 32000, SDS-PAGE Synechocystis sp.
70000
-
gel filtration Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme expressed in Escherichia coli Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
88.1
-
-
Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate + NADP+
-
Synechocystis sp. L-tyrosine + NADPH + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
TyrAsy
-
Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Synechocystis sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 enzyme activity dropps below pH 6.5 and above pH 8.5 Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
NADP+ no activity with NAD+ Synechocystis sp.