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Literature summary for 1.3.1.77 extracted from

  • Zhang, X.; Liu, Y.; Gao, K.; Zhao, L.; Liu, L.; Wang, Y.; Sun, M.; Gao, L.; Xia, T.
    Characterisation of anthocyanidin reductase from Shuchazao green tea (2012), J. Sci. Food Agric., 92, 1533-1539.
    View publication on PubMed

Application

Application Comment Organism
molecular biology a method for the analysis of ANR activity using the detection of coenzyme is established Camellia sinensis

Organism

Organism UniProt Comment Textmining
Camellia sinensis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Camellia sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanidin + NADPH + H+ changes in the concentration of products and coenzyme in the ANR assay are determined by thin layer chromatography (TLC), HPLC, mass spectrometry (MS) and UV spectrophotometry Camellia sinensis (-)-epicatechin + NADP+
-
?
delphinidin + NADPH changes in the concentration of products and coenzyme in the ANR assay are determined by thin layer chromatography (TLC), HPLC, mass spectrometry (MS) and UV spectrophotometry Camellia sinensis (-)-epigallocatechin + NAD(P)+
-
?

Synonyms

Synonyms Comment Organism
ANR
-
Camellia sinensis
anthocyanidin reductase
-
Camellia sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
assay at Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Camellia sinensis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Camellia sinensis

Expression

Organism Comment Expression
Camellia sinensis gene expression and enzymatic activity are higher in the developping leaves than in the mature leaves and lower than in the tender bud and first leaves up