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Literature summary for 1.3.1.76 extracted from

  • Raux, E.; Leech, H.K.; Beck, R.; Schubert, H.L.; Santander, P.J.; Roessner, C.A.; Scott, A.I.; Martens, J.H.; Jahn, D.; Thermes, C.; Rambach, A.; Warren, M.J.
    Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium (2003), Biochem. J., 370, 505-516.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
sequenced and overproduced, sirABC gene cluster complements Escherichia coli cysG mutants Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 2 * 22000, homodimer, SDS-PAGE Priestia megaterium
45000
-
 gel filtration Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
precorrin-2 + NAD+ Priestia megaterium siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III sirohydrochlorin + NADH + H+
-
 ?
precorrin-2 + NAD+ Priestia megaterium DSM 509 siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III sirohydrochlorin + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Priestia megaterium P61818 DSM509
-
Priestia megaterium DSM 509 P61818 DSM509
-

Purification (Commentary)

Purification (Comment) Organism
-
 Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
-
 Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
precorrin-2 + NAD+ unlike Met8p and CysG, SirC has no chelatase activity Priestia megaterium sirohydrochlorin + NADH + H+
-
 ?
precorrin-2 + NAD+ siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III Priestia megaterium sirohydrochlorin + NADH + H+
-
 ?
precorrin-2 + NAD+ unlike Met8p and CysG, SirC has no chelatase activity Priestia megaterium DSM 509 sirohydrochlorin + NADH + H+
-
 ?
precorrin-2 + NAD+ siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III Priestia megaterium DSM 509 sirohydrochlorin + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 22000, homodimer, SDS-PAGE Priestia megaterium

Synonyms

Synonyms Comment Organism
precorrin-2 dehydrogenase
-
 Priestia megaterium
SirC
-
 Priestia megaterium