Cloned (Comment) | Organism |
---|---|
gene bchXYZa | Rhodopseudomonas palustris |
gene bchXYZa, cloning and DNA and amino acid sequence determination and analysis, the plasmid carrying genes bchXYZ is used for complementation fo bchXYZ-deficient mutant strains of Rhodobacter sphaeroides, Rhodobacter palustris, Chlorobium tepidum, and Rhodobacter castenholzii | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a bchXYZ deletion mutant, construction of a Rhodobacter palustris bciA mutant. The mutant lacking sole BciA can grow under anaerobic light conditions as well as aerobic dark conditions. On the other hand, the single bchXYZ mutant and the double bciA/bchXYZ mutant do not show phototrophic growth and grow under aerobic/semiaerobic conditions in the dark | Cereibacter sphaeroides |
additional information | construction of a bchXYZ deletion mutant, construction of a Rhodobacter palustris bciB mutant using the plasmid pJSC-palB-Km | Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + NADP+ | Cereibacter sphaeroides | - |
divinyl chlorophyllide a + NADPH + H+ | - |
? | |
chlorophyllide a + NADP+ | Rhodopseudomonas palustris | - |
divinyl chlorophyllide a + NADPH + H+ | - |
? | |
chlorophyllide a + NADP+ | Cereibacter sphaeroides J001 | - |
divinyl chlorophyllide a + NADPH + H+ | - |
? | |
chlorophyllide a + NADP+ | Rhodopseudomonas palustris J1002 | - |
divinyl chlorophyllide a + NADPH + H+ | - |
? | |
chlorophyllide a + NADP+ | Rhodopseudomonas palustris J002 | - |
divinyl chlorophyllide a + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
rifampicin-resistant mutant strain J001 derived from wild-type strain 2.4.1, gene bchXYZ | - |
Cereibacter sphaeroides J001 | - |
rifampicin-resistant mutant strain J001 derived from wild-type strain 2.4.1, gene bchXYZ | - |
Rhodopseudomonas palustris | - |
a rifampicin-resistant variant of wild-type strain CGA009, gene bciB | - |
Rhodopseudomonas palustris J002 | - |
a rifampicin-resistant variant of wild-type strain CGA009, gene bciB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + NADP+ | - |
Cereibacter sphaeroides | divinyl chlorophyllide a + NADPH + H+ | - |
? | |
chlorophyllide a + NADP+ | - |
Rhodopseudomonas palustris | divinyl chlorophyllide a + NADPH + H+ | - |
? | |
chlorophyllide a + NADP+ | - |
Cereibacter sphaeroides J001 | divinyl chlorophyllide a + NADPH + H+ | - |
? | |
chlorophyllide a + NADP+ | - |
Rhodopseudomonas palustris J1002 | divinyl chlorophyllide a + NADPH + H+ | - |
? | |
chlorophyllide a + NADP+ | - |
Rhodopseudomonas palustris J002 | divinyl chlorophyllide a + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BciA | - |
Cereibacter sphaeroides |
bciB | - |
Rhodopseudomonas palustris |
COR | - |
Cereibacter sphaeroides |
divinyl-chlorophyllide a reductase | - |
Cereibacter sphaeroides |
divinyl-chlorophyllide a reductase | - |
Rhodopseudomonas palustris |
DVR | - |
Cereibacter sphaeroides |
DVR | - |
Rhodopseudomonas palustris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Cereibacter sphaeroides | |
NADP+ | - |
Rhodopseudomonas palustris |
General Information | Comment | Organism |
---|---|---|
malfunction | single mutants with deleted BciA or COR show production of the C8 ethyl group pigments, whereas the double mutant accumulates 8-vinyl-chlorophyllide, indicating that there is no enzyme other than BciA and COR functioning as the third DVR in Rhodobacter sphaeroides, which has no bciB gene. COR genes derived from other groups of anoxygenic photosynthetic bacteria and introduced into the double mutant all complement the organism and produce normal bacteriochlorophyll a | Rhodopseudomonas palustris |
malfunction | single mutants with deleted BciA or COR show production of the C8 ethyl group pigments, whereas the double mutant accumulates 8-vinyl-chlorophyllide, indicating that there is no enzyme other than BciA and COR functioning as the third DVR in Rhodobacter sphaeroides, which has no bciB gene. COR genes derived from other groups of anoxygenic photosynthetic bacteria and introduced into the double mutant all complement the organism and produce normal bacteriochlorophyll a, pigment compositions of wild-type and mutant strains, overview | Cereibacter sphaeroides |
metabolism | in bacteriochlorophyll a biosynthesis, the reduction of the C8 vinyl group in 8-vinyl-chlorophyllide a is catalyzed to produce chlorophyllide a by an 8-vinyl reductase called divinyl reductase (DVR), which is divided into two types, BciA and BciB. Rhodopseudomonas palustris has only BciB as its divinyl reductase DVR. Chlorophyllide a is converted into 3-vinylbacteriochlorophyllide a and then into bacteriochlorophyll a, pathway overview | Rhodopseudomonas palustris |
metabolism | in bacteriochlorophyll a biosynthesis, the reduction of the C8 vinyl group in 8-vinyl-chlorophyllide a is catalyzed to produce chlorophyllide a by an 8-vinyl reductase called divinyl reductase (DVR), which is into two types, BciA and BciB. Rhodobacter sphaeroides contains BciA but no BciB, and also another distinct divinyl reductase. Chlorophyllide a oxidoreductase, COR, performs two functions: reductions of the C8 vinyl group and the C7=C8 double bond. Chlorophyllide a is converted into 3-vinylbacteriochlorophyllide a and then into bacteriochlorophyll a, pathway overview | Cereibacter sphaeroides |