Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli as a GST-fusion protein | Arabidopsis thaliana |
overexpressed in Escherichia coli as a GST-fusion protein | Cucumis sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | pH 7.0, 25°C | Arabidopsis thaliana | |
0.023 | - |
4-hexen-3-one | pH 7.0, 25°C | Cucumis sativus | |
0.053 | - |
NADH | pH 7.0, 25°C | Arabidopsis thaliana | |
0.12 | - |
4-hexen-3-one | pH 7.0, 25°C | Arabidopsis thaliana | |
0.13 | - |
3-buten-2-one | pH 7.0, 25°C | Arabidopsis thaliana | |
0.185 | - |
NADPH | pH 7.0, 25°C | Cucumis sativus | |
0.29 | - |
1-penten-3-one | pH 7.0, 25°C | Arabidopsis thaliana | |
0.58 | - |
1-penten-3-one | pH 7.0, 25°C | Cucumis sativus | |
0.63 | - |
3-penten-2-one | pH 7.0, 25°C | Cucumis sativus | |
1.8 | - |
crotonaldehyde | pH 7.0, 25°C | Arabidopsis thaliana | |
2.88 | - |
3-buten-2-one | pH 7.0, 25°C | Cucumis sativus | |
3.5 | - |
propenal | pH 7.0, 25°C | Arabidopsis thaliana | |
4.64 | - |
NADH | pH 7.0, 25°C | Cucumis sativus | |
13.6 | - |
propenal | pH 7.0, 25°C | Cucumis sativus | |
29.8 | - |
crotonaldehyde | pH 7.0, 25°C | Cucumis sativus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
chloroplast | - |
Cucumis sativus | 9507 | - |
cytosol | - |
Cucumis sativus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
SDS-PAGE | Arabidopsis thaliana |
42000 | - |
SDS-PAGE | Cucumis sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Cucumis sativus | E2S0A5 | chloroplast variant | - |
Cucumis sativus | E2S0A6 | cytosolic variant | - |
Purification (Comment) | Organism |
---|---|
using anion exchange chromatography, hydroxyapatite, phenyl-Sepharose, and gel filtration | Arabidopsis thaliana |
using anion exchange chromatography, hydroxyapatite, phenyl-Sepharose, and gel filtration | Cucumis sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
leaf | - |
Cucumis sativus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-penten-3-one + NADH | - |
Arabidopsis thaliana | ? + NAD+ | - |
? | |
1-penten-3-one + NADH | - |
Cucumis sativus | ? + NAD+ | - |
? | |
1-penten-3-one + NADH | - |
Cucumis sativus | pentan-3-one + NAD+ | - |
? | |
1-penten-3-one + NADPH | - |
Cucumis sativus | ? + NADP+ | - |
? | |
1-penten-3-one + NADPH | - |
Arabidopsis thaliana | pentan-3-one + NADP+ | - |
? | |
1-penten-3-one + NADPH | - |
Cucumis sativus | pentan-3-one + NADP+ | - |
? | |
3-buten-2-one + NADH | - |
Arabidopsis thaliana | 2-butanone + NAD+ | - |
? | |
3-buten-2-one + NADH | - |
Cucumis sativus | 2-butanone + NAD+ | - |
? | |
3-buten-2-one + NADPH | - |
Arabidopsis thaliana | 2-butanone + NADP+ | - |
? | |
3-buten-2-one + NADPH | - |
Cucumis sativus | 2-butanone + NADP+ | - |
? | |
3-penten-2-one + NADH | - |
Cucumis sativus | 2-pentanone + NAD+ | - |
? | |
3-penten-2-one + NADPH | - |
Cucumis sativus | 2-pentanone + NADP+ | - |
? | |
4-hexen-3-one + NADH | - |
Arabidopsis thaliana | hexan-3-one + NAD+ | - |
? | |
4-hexen-3-one + NADH | - |
Cucumis sativus | hexan-3-one + NAD+ | - |
? | |
4-hexen-3-one + NADPH | - |
Arabidopsis thaliana | hexan-3-one + NADP+ | - |
? | |
4-hexen-3-one + NADPH | - |
Cucumis sativus | hexan-3-one + NADP+ | - |
? | |
crotonaldehyde + NADH | - |
Arabidopsis thaliana | butanal + NAD+ | - |
? | |
crotonaldehyde + NADH | - |
Cucumis sativus | butanal + NAD+ | - |
? | |
crotonaldehyde + NADPH | - |
Arabidopsis thaliana | butanal + NADP+ | - |
? | |
crotonaldehyde + NADPH | - |
Cucumis sativus | butanal + NADP+ | - |
? | |
propenal + NADH | - |
Arabidopsis thaliana | propanal + NAD+ | - |
? | |
propenal + NADH | - |
Cucumis sativus | propanal + NAD+ | - |
? | |
propenal + NADPH | - |
Arabidopsis thaliana | propanal + NADP+ | - |
? | |
propenal + NADPH | - |
Cucumis sativus | propanal + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Arabidopsis thaliana |
monomer | - |
Cucumis sativus |
Synonyms | Comment | Organism |
---|---|---|
alkenal/one oxidoreductase | - |
Arabidopsis thaliana |
alkenal/one oxidoreductase | - |
Cucumis sativus |
AtAOR | - |
Arabidopsis thaliana |
CsAOR | - |
Cucumis sativus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
crotonaldehyde | pH 7.0, 25°C | Arabidopsis thaliana | |
3.3 | - |
4-hexen-3-one | pH 7.0, 25°C | Arabidopsis thaliana | |
3.5 | - |
3-buten-2-one | pH 7.0, 25°C | Arabidopsis thaliana | |
4 | - |
1-penten-3-one | pH 7.0, 25°C | Arabidopsis thaliana | |
22 | - |
3-penten-2-one | pH 7.0, 25°C | Cucumis sativus | |
22 | - |
propenal | pH 7.0, 25°C | Arabidopsis thaliana | |
23 | - |
NADPH | pH 7.0, 25°C | Arabidopsis thaliana | |
23 | - |
NADH | pH 7.0, 25°C | Arabidopsis thaliana | |
42 | - |
crotonaldehyde | pH 7.0, 25°C | Cucumis sativus | |
61 | - |
1-penten-3-one | pH 7.0, 25°C | Cucumis sativus | |
63 | - |
propenal | pH 7.0, 25°C | Cucumis sativus | |
68 | - |
4-hexen-3-one | pH 7.0, 25°C | Cucumis sativus | |
74 | - |
3-buten-2-one | pH 7.0, 25°C | Cucumis sativus | |
75 | - |
NADPH | pH 7.0, 25°C | Cucumis sativus | |
75 | - |
NADH | pH 7.0, 25°C | Cucumis sativus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | enzyme operates 30times faster in the presence of NADPH than in the presence of NADH | Arabidopsis thaliana | |
NADH | enzyme operates 30times faster in the presence of NADPH than in the presence of NADH | Cucumis sativus | |
NADPH | enzyme operates 30times faster in the presence of NADPH than in the presence of NADH | Arabidopsis thaliana | |
NADPH | enzyme operates 30times faster in the presence of NADPH than in the presence of NADH | Cucumis sativus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.63 | - |
propenal | pH 7.0, 25°C | Cucumis sativus | |
6.28 | - |
propenal | pH 7.0, 25°C | Arabidopsis thaliana | |
13.8 | - |
1-penten-3-one | pH 7.0, 25°C | Arabidopsis thaliana | |
16.2 | - |
NADH | pH 7.0, 25°C | Cucumis sativus | |
25.7 | - |
3-buten-2-one | pH 7.0, 25°C | Cucumis sativus | |
26.9 | - |
3-buten-2-one | pH 7.0, 25°C | Arabidopsis thaliana | |
27.5 | - |
4-hexen-3-one | pH 7.0, 25°C | Arabidopsis thaliana | |
34.9 | - |
3-penten-2-one | pH 7.0, 25°C | Cucumis sativus | |
105 | - |
1-penten-3-one | pH 7.0, 25°C | Cucumis sativus | |
406 | - |
NADPH | pH 7.0, 25°C | Cucumis sativus | |
434 | - |
NADH | pH 7.0, 25°C | Arabidopsis thaliana | |
2300 | - |
NADPH | pH 7.0, 25°C | Arabidopsis thaliana | |
2960 | - |
4-hexen-3-one | pH 7.0, 25°C | Cucumis sativus |