Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucurbita maxima | - |
var. Green Hubbard | - |
Purification (Comment) | Organism |
---|---|
- |
Cucurbita maxima |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+ | plant terpenoid | Cucurbita maxima |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Cucurbita maxima | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5812 | - |
- |
Cucurbita maxima |
Storage Stability | Organism |
---|---|
0-4°C, maleic acid-NaOH buffer, pH 6.6, 100 mM NaCl, 5 weeks | Cucurbita maxima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cucurbitacin B + NAD(P)H + H+ | - |
Cucurbita maxima | 23,24-dihydrocucurbitacin B + NAD(P)+ | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cucurbita maxima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, no loss of activity, 10 min, 30% loss of activity | Cucurbita maxima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.65 | - |
crude enzyme preparation in presence of Zn2+ has a wider pH optimum range than purified enzyme in presence of Mn2+ | Cucurbita maxima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 25% of activity at maximum, pH 8.0: about 80% of activity at maximum of crude enzyme preparation in presence of Zn2+, about 10% of activity at maximum of purified enzyme in presence of Mn2+ | Cucurbita maxima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Cucurbita maxima |