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Literature summary for 1.3.1.5 extracted from

  • Schabort, J.C.; Potgieter, D.J.J.; De Villiers, V.
    Cucurbitacin B DELTA23-reductase from Cucurbita maxima. I. Assay methods, isolation and purification (1968), Biochim. Biophys. Acta, 151, 33-46.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Cucurbita maxima
-
var. Green Hubbard
-

Purification (Commentary)

Purification (Comment) Organism
-
Cucurbita maxima

Reaction

Reaction Comment Organism Reaction ID
23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+ plant terpenoid Cucurbita maxima

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Cucurbita maxima
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5812
-
-
Cucurbita maxima

Storage Stability

Storage Stability Organism
0-4°C, maleic acid-NaOH buffer, pH 6.6, 100 mM NaCl, 5 weeks Cucurbita maxima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cucurbitacin B + NAD(P)H + H+
-
Cucurbita maxima 23,24-dihydrocucurbitacin B + NAD(P)+
-
ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Cucurbita maxima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
5 min, no loss of activity, 10 min, 30% loss of activity Cucurbita maxima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.65
-
crude enzyme preparation in presence of Zn2+ has a wider pH optimum range than purified enzyme in presence of Mn2+ Cucurbita maxima

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 25% of activity at maximum, pH 8.0: about 80% of activity at maximum of crude enzyme preparation in presence of Zn2+, about 10% of activity at maximum of purified enzyme in presence of Mn2+ Cucurbita maxima

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
Cucurbita maxima