Literature summary for 1.3.1.48 extracted from

Kitamura, S.; Katsura, H.; Tatsumi, K.
Purification of a 15-ketoprostaglandin DELTA13-reductase from rat liver and its ability to reduce the double bond of xenobiotics (1993), Biochem. Mol. Biol. Int., 30, 839-847.

Inhibitors

Inhibitors	Comment	Organism
dicoumarol	0.1 mM, complete inhibition	Rattus norvegicus
indomethacin	0.1 mM, 95% inhibition	Rattus norvegicus
p-chloromercuribenzoate	0.1 mM, 97% inhibition	Rattus norvegicus
quercitrin	95% inhibition	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000213	-	15-ketoprostaglandin F2alpha	-	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39500	-	1 * 39500, SDS-PAGE	Rattus norvegicus
42000	-	gel filtration	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-cellulose, phenyl-toyopearl, blue-toyopearl, hydroxyapatite, liver enzyme	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
adrenal gland	-	Rattus norvegicus	-
brain	-	Rattus norvegicus	-
heart	-	Rattus norvegicus	-
kidney	-	Rattus norvegicus	-
liver	-	Rattus norvegicus	-
lung	-	Rattus norvegicus	-
pancreas	-	Rattus norvegicus	-
small intestine	-	Rattus norvegicus	-
spleen	-	Rattus norvegicus	-
testis	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.05	-	-	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H	-	Rattus norvegicus	(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+	-	?
(Z)-3-[(5-methoxy-3-oxo-2(3H)-benzofuranylidene)-methyl]benzoic acid + NAD(P)H	-	Rattus norvegicus	3-[(5-methoxy-3-oxo-2,3-dihydro-1-benzofuran-2-yl)methyl]benzoic acid + NAD(P)+	-	?
1-(p-carboxyphenyl)-5-cyano-3-oxo-1-pentene + NAD(P)H	-	Rattus norvegicus	1-(p-carboxyphenyl)-5-cyano-3-oxo-pentane + NAD(P)+	-	?
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H	-	Rattus norvegicus	11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+	-	?
15-ketoprostaglandin F1alpha + NAD(P)H	-	Rattus norvegicus	?	-	?
15-ketoprostaglandin F2alpha + NADPH	-	Rattus norvegicus	?	-	?
beta-ionone + NAD(P)H	-	Rattus norvegicus	?	-	?
curcumin + NAD(P)H	-	Rattus norvegicus	?	-	?
trans-2-(o-hydroxybenzylidene)acetophenone + NAD(P)H	-	Rattus norvegicus	(o-hydroxybenzyl)acetophenone + NAD(P)+	-	?
trans-2-benzylidenecyclohexan-2-one + NAD(P)H	-	Rattus norvegicus	benzylcyclohexan-2-one + NAD(P)+	-	?
trans-2-butenal + NAD(P)H	-	Rattus norvegicus	butanal + NAD(P)+	-	?
trans-2-hexenal + NAD(P)H	-	Rattus norvegicus	hexanal + NAD(P)+	-	?
trans-3-(2-furyl)acrolein + NAD(P)H	-	Rattus norvegicus	2-furylpropanal + NAD(P)+	-	?
trans-4-furyl-3-buten-2-one + NAD(P)H	-	Rattus norvegicus	4-furyl-butane-2-one + NAD(P)+	-	?
trans-benzylideneacetone + NAD(P)H	-	Rattus norvegicus	benzylacetone + NAD(P)+	-	?
trans-benzylideneacetophenone + NAD(P)H	-	Rattus norvegicus	benzylacetophenone + NAD(P)+	-	?
trans-cinnamaldehyde + NAD(P)H	-	Rattus norvegicus	?	-	?
trans-cinnamic acid ethyl ester + NAD(P)H	-	Rattus norvegicus	?	-	?
trans-cinnamoyl thiophene + NAD(P)H	-	Rattus norvegicus	?	-	?
trans-methylvinylketone + NAD(P)H	-	Rattus norvegicus	butane-2-one + NAD(P)+	-	?
trans-phenyl-1-propenyl ketone + NAD(P)H	-	Rattus norvegicus	phenylpropane-1-one + NAD+	-	?
trans-trans-2,4-hexadienal + NAD(P)H	-	Rattus norvegicus	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 39500, SDS-PAGE	Rattus norvegicus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8	-	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
NAD(P)H	in crude extracts: NADH is 30% as effective as NADPH, in purified enzyme: NADH is 10% as effectic as NADPH	Rattus norvegicus