KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
Biochanin A | - |
Fusarium solani-melongenae | |
0.19 | - |
NADPH | - |
Fusarium solani-melongenae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Fusarium solani-melongenae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | 88000 | gel filtration | Fusarium solani-melongenae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+ | Fusarium solani-melongenae | trivial name biochanin A | (3S)-2-dihydrobiochanin A + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium solani-melongenae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Fusarium solani-melongenae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.355 | - |
- |
Fusarium solani-melongenae |
Storage Stability | Organism |
---|---|
-25°C, 20% v/v glycerol, with NADPH, but unstable with NADP+ | Fusarium solani-melongenae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,7,3'-trihydroxy-4'-methoxyisoflavone + NADPH + H+ | trivial name pratensein, 78% of activity with biochanin A | Fusarium solani-melongenae | 2-dihydropratensein + NADP+ | - |
? | |
5,7,3'-trihydroxyisoflavone + NADPH + H+ | trivial name genistein, 44% of activity with biochanin A | Fusarium solani-melongenae | 2-dihydrogenistein + NADP+ | - |
? | |
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+ | highly specific for 5,7-hydroxy group and some specificity for 4'-substituent of isoflavones, no reaction with flavones, chalcones, 5-deoxyisoflavones | Fusarium solani-melongenae | (3S)-2-dihydrobiochanin A + NADP+ | hydrated at 2 position or ring A of isoflavone | ? | |
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+ | trivial name biochanin A | Fusarium solani-melongenae | (3S)-2-dihydrobiochanin A + NADP+ | - |
? | |
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+ | trivial name biochanin A | Fusarium solani-melongenae | (3S)-2-dihydrobiochanin A + NADP+ | hydrated at 2 position or ring A of isoflavone | ? | |
biochanin A + NADPH + H+ | - |
Fusarium solani-melongenae | dihydrobiochanin A + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000-44000, SDS-PAGE | Fusarium solani-melongenae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Fusarium solani-melongenae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
inactivation above | Fusarium solani-melongenae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Fusarium solani-melongenae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Fusarium solani-melongenae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | 30% of maximum activity with NADH | Fusarium solani-melongenae |