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Literature summary for 1.3.1.45 extracted from

  • Brandalise, M.; Severino, F.E.; Maluf, M.P.; Maia, I.G.
    The promoter of a gene encoding an isoflavone reductase-like protein in coffee (Coffea arabica) drives a stress-responsive expression in leaves (2009), Plant Cell Rep., 28, 1699-1708.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene constructs lacking the promoter of CaIRL are introduced into Agrobacterium tumefaciens strain LBA4404 and used to transform tobacco leaf discs Coffea arabica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
predicted from nucleotide sequence encoding 314 amino acids Coffea arabica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-hydroxyformononetin + NADPH + H+ Coffea arabica CaIRL protein is a new member of the PIP family of NADPH-dependent reductases, enzymes are involved in the biosynthesis of plant defense metabolites such as lignans and isoflavonoids vestitone + NADP+
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?

Organism

Organism UniProt Comment Textmining
Coffea arabica C7BFZ4 variant Mundo Novo (IAC 388-17-1)
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Source Tissue

Source Tissue Comment Organism Textmining
leaf specific Coffea arabica
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additional information no expression in root, stem and flower Coffea arabica
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
a 5'-deletion to -404 completely abolishes promoter activation by abiotic stimulus in transgenic plants Coffea arabica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-hydroxyformononetin + NADPH + H+ CaIRL protein is a new member of the PIP family of NADPH-dependent reductases, enzymes are involved in the biosynthesis of plant defense metabolites such as lignans and isoflavonoids Coffea arabica vestitone + NADP+
-
?

Synonyms

Synonyms Comment Organism
CaIRL
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Coffea arabica
IFR
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Coffea arabica
isoflavone reductase
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Coffea arabica
isoflavone reductase-like protein
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Coffea arabica

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Coffea arabica

Expression

Organism Comment Expression
Coffea arabica the full-length version of the CaIRL promoter directs a reporter gene expression that is stimulated by mechanical injury as the expression from the native CaIRL gene up
Coffea arabica the promoter of a gene encoding an isoflavone reductase-like protein in coffee drives a stress-responsive expression in leaves up
Coffea arabica transcript level is markedly increased in response to fungal infection and mechanical injury, a rapid and marked increase in CaIRL expression is also observed following mechanical injury of the leaves (75fold after 4 h treatment), this induction is followed by a progressive decrease in transcript accumulation at 8 and 12 h post injury, the activation of CaIRL expression by mechanical injury is faster than that observed in response to fungal inoculation up