Cloned (Comment) | Organism |
---|---|
gene constructs lacking the promoter of CaIRL are introduced into Agrobacterium tumefaciens strain LBA4404 and used to transform tobacco leaf discs | Coffea arabica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
predicted from nucleotide sequence encoding 314 amino acids | Coffea arabica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-hydroxyformononetin + NADPH + H+ | Coffea arabica | CaIRL protein is a new member of the PIP family of NADPH-dependent reductases, enzymes are involved in the biosynthesis of plant defense metabolites such as lignans and isoflavonoids | vestitone + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea arabica | C7BFZ4 | variant Mundo Novo (IAC 388-17-1) | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | specific | Coffea arabica | - |
additional information | no expression in root, stem and flower | Coffea arabica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
a 5'-deletion to -404 completely abolishes promoter activation by abiotic stimulus in transgenic plants | Coffea arabica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-hydroxyformononetin + NADPH + H+ | CaIRL protein is a new member of the PIP family of NADPH-dependent reductases, enzymes are involved in the biosynthesis of plant defense metabolites such as lignans and isoflavonoids | Coffea arabica | vestitone + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CaIRL | - |
Coffea arabica |
IFR | - |
Coffea arabica |
isoflavone reductase | - |
Coffea arabica |
isoflavone reductase-like protein | - |
Coffea arabica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Coffea arabica |
Organism | Comment | Expression |
---|---|---|
Coffea arabica | the full-length version of the CaIRL promoter directs a reporter gene expression that is stimulated by mechanical injury as the expression from the native CaIRL gene | up |
Coffea arabica | the promoter of a gene encoding an isoflavone reductase-like protein in coffee drives a stress-responsive expression in leaves | up |
Coffea arabica | transcript level is markedly increased in response to fungal infection and mechanical injury, a rapid and marked increase in CaIRL expression is also observed following mechanical injury of the leaves (75fold after 4 h treatment), this induction is followed by a progressive decrease in transcript accumulation at 8 and 12 h post injury, the activation of CaIRL expression by mechanical injury is faster than that observed in response to fungal inoculation | up |