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Literature summary for 1.3.1.43 extracted from

  • Xie, G.; Bonner, C.A.; Jensen, R.A.
    Cyclohexadienyl dehydrogenase from Pseudomonas stutzeri exemplifies a widespread type of tyrosine-pathway dehydrogenase in the TyrA protein family (2000), Comp. Biochem. Physiol. C, 125, 65-83.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
tyrAc gene, sequence analysis, overexpression of His-tagged protein in Escherichia coli Pseudomonas stutzeri

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxyphenylalanine slight inhibition Pseudomonas stutzeri
4-amino-L-phenylalanine
-
Pseudomonas stutzeri
4-hydroxybenzoate
-
Pseudomonas stutzeri
4-hydroxyphenylacetate
-
Pseudomonas stutzeri
4-hydroxyphenylpyruvate
-
Pseudomonas stutzeri
D-tyrosine
-
Pseudomonas stutzeri
DL-4-hydroxyphenyllactate
-
Pseudomonas stutzeri
L-phenylalanine slight inhibition Pseudomonas stutzeri
L-tyrosine competitive inhibition Pseudomonas stutzeri
m-Fluoro-DL-tyrosine
-
Pseudomonas stutzeri
additional information no inhibition with phenyllactate and benzoate Pseudomonas stutzeri
phenol
-
Pseudomonas stutzeri
phenylpyruvate no inhibition Pseudomonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
prephenate
-
Pseudomonas stutzeri
0.14
-
NAD+
-
Pseudomonas stutzeri
0.56
-
L-arogenate
-
Pseudomonas stutzeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
2 * 33000, recombinant His-tagged protein, forms a tetramer at high protein concentration, SDS-PAGE Pseudomonas stutzeri
66000
-
dimer, gel filtration Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arogenate + NAD+ Pseudomonas stutzeri
-
L-tyrosine + NADH + CO2
-
?
L-arogenate + NAD+ Pseudomonas stutzeri JM300
-
L-tyrosine + NADH + CO2
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
JM300
-
Pseudomonas stutzeri JM300
-
JM300
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged protein from Escherichia coli Pseudomonas stutzeri

Reaction

Reaction Comment Organism Reaction ID
L-arogenate + NAD+ = L-tyrosine + NADH + CO2 + H+ TyrA dehydrogenase superfamily Pseudomonas stutzeri
L-arogenate + NAD+ = L-tyrosine + NADH + CO2 + H+ substrate binding and inhibitory mechanism Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate + NAD(P)+
-
Pseudomonas stutzeri L-tyrosine + NAD(P)H + CO2
-
?
L-arogenate + NAD(P)+
-
Pseudomonas stutzeri JM300 L-tyrosine + NAD(P)H + CO2
-
?
L-arogenate + NAD+
-
Pseudomonas stutzeri L-tyrosine + NADH + CO2
-
?
L-arogenate + NAD+
-
Pseudomonas stutzeri JM300 L-tyrosine + NADH + CO2
-
?
additional information activity with prephenate and L-arogenate is unseparable during purification, thus cyclohexadienyl dehydrogenase activity Pseudomonas stutzeri ?
-
?
additional information activity with prephenate and L-arogenate is unseparable during purification, thus cyclohexadienyl dehydrogenase activity Pseudomonas stutzeri JM300 ?
-
?
prephenate + NAD(P)+
-
Pseudomonas stutzeri ?
-
?
prephenate + NAD(P)+
-
Pseudomonas stutzeri JM300 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 33000, recombinant His-tagged protein, forms a tetramer at high protein concentration, SDS-PAGE Pseudomonas stutzeri

Synonyms

Synonyms Comment Organism
cyclohexadienyl dehydrogenase
-
Pseudomonas stutzeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas stutzeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
-
Pseudomonas stutzeri

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
Pseudomonas stutzeri

Cofactor

Cofactor Comment Organism Structure
NAD+ only weak activity with NADP+, 0.25% of activity with NAD+ Pseudomonas stutzeri

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.23
-
4-hydroxylphenylpyruvate
-
Pseudomonas stutzeri
0.26
-
L-tyrosine competitive inhibition Pseudomonas stutzeri