Cloned (Comment) | Organism |
---|---|
tyrAc gene, sequence analysis, overexpression of His-tagged protein in Escherichia coli | Pseudomonas stutzeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dihydroxyphenylalanine | slight inhibition | Pseudomonas stutzeri | |
4-amino-L-phenylalanine | - |
Pseudomonas stutzeri | |
4-hydroxybenzoate | - |
Pseudomonas stutzeri | |
4-hydroxyphenylacetate | - |
Pseudomonas stutzeri | |
4-hydroxyphenylpyruvate | - |
Pseudomonas stutzeri | |
D-tyrosine | - |
Pseudomonas stutzeri | |
DL-4-hydroxyphenyllactate | - |
Pseudomonas stutzeri | |
L-phenylalanine | slight inhibition | Pseudomonas stutzeri | |
L-tyrosine | competitive inhibition | Pseudomonas stutzeri | |
m-Fluoro-DL-tyrosine | - |
Pseudomonas stutzeri | |
additional information | no inhibition with phenyllactate and benzoate | Pseudomonas stutzeri | |
phenol | - |
Pseudomonas stutzeri | |
phenylpyruvate | no inhibition | Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
prephenate | - |
Pseudomonas stutzeri | |
0.14 | - |
NAD+ | - |
Pseudomonas stutzeri | |
0.56 | - |
L-arogenate | - |
Pseudomonas stutzeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, recombinant His-tagged protein, forms a tetramer at high protein concentration, SDS-PAGE | Pseudomonas stutzeri |
66000 | - |
dimer, gel filtration | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arogenate + NAD+ | Pseudomonas stutzeri | - |
L-tyrosine + NADH + CO2 | - |
? | |
L-arogenate + NAD+ | Pseudomonas stutzeri JM300 | - |
L-tyrosine + NADH + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | - |
JM300 | - |
Pseudomonas stutzeri JM300 | - |
JM300 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein from Escherichia coli | Pseudomonas stutzeri |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-arogenate + NAD+ = L-tyrosine + NADH + CO2 + H+ | TyrA dehydrogenase superfamily | Pseudomonas stutzeri | |
L-arogenate + NAD+ = L-tyrosine + NADH + CO2 + H+ | substrate binding and inhibitory mechanism | Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arogenate + NAD(P)+ | - |
Pseudomonas stutzeri | L-tyrosine + NAD(P)H + CO2 | - |
? | |
L-arogenate + NAD(P)+ | - |
Pseudomonas stutzeri JM300 | L-tyrosine + NAD(P)H + CO2 | - |
? | |
L-arogenate + NAD+ | - |
Pseudomonas stutzeri | L-tyrosine + NADH + CO2 | - |
? | |
L-arogenate + NAD+ | - |
Pseudomonas stutzeri JM300 | L-tyrosine + NADH + CO2 | - |
? | |
additional information | activity with prephenate and L-arogenate is unseparable during purification, thus cyclohexadienyl dehydrogenase activity | Pseudomonas stutzeri | ? | - |
? | |
additional information | activity with prephenate and L-arogenate is unseparable during purification, thus cyclohexadienyl dehydrogenase activity | Pseudomonas stutzeri JM300 | ? | - |
? | |
prephenate + NAD(P)+ | - |
Pseudomonas stutzeri | ? | - |
? | |
prephenate + NAD(P)+ | - |
Pseudomonas stutzeri JM300 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, recombinant His-tagged protein, forms a tetramer at high protein concentration, SDS-PAGE | Pseudomonas stutzeri |
Synonyms | Comment | Organism |
---|---|---|
cyclohexadienyl dehydrogenase | - |
Pseudomonas stutzeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas stutzeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Pseudomonas stutzeri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Pseudomonas stutzeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | only weak activity with NADP+, 0.25% of activity with NAD+ | Pseudomonas stutzeri |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
4-hydroxylphenylpyruvate | - |
Pseudomonas stutzeri | |
0.26 | - |
L-tyrosine | competitive inhibition | Pseudomonas stutzeri |