KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0283 | - |
(15Z)-12-oxophyto-10,15-dienoate | pH 7.2, temperature not specified in the publication | Arabidopsis thaliana | |
0.0385 | - |
Methyl vinyl ketone | pH 7.2, temperature not specified in the publication | Arabidopsis thaliana | |
1.967 | - |
2-methylcyclopent-2-en-1-one | pH 7.2, temperature not specified in the publication | Arabidopsis thaliana | |
4.36 | - |
trans-hex-2-enal | pH 7.2, temperature not specified in the publication | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FUP0 | cf. EC 1.6.5.4 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
substrate maleic acid, pH 7.2, temperature not specified in the publication | Arabidopsis thaliana |
0.6 | - |
substrate trans-hex-2-enal, pH 7.2, temperature not specified in the publication | Arabidopsis thaliana |
2 | - |
substrate (15Z)-12-oxophyto-10,15-dienoate, pH 7.2, temperature not specified in the publication | Arabidopsis thaliana |
2.5 | - |
substrate 2-methylcyclopent-2-en-1-one, pH 7.2, temperature not specified in the publication | Arabidopsis thaliana |
13.5 | - |
substrate methyl vinyl ketone, pH 7.2, temperature not specified in the publication | Arabidopsis thaliana |
22.8 | - |
substrate N-ethyl-maleimide, pH 7.2, temperature not specified in the publication | Arabidopsis thaliana |
26.9 | - |
substrate 1,4-benzoquinone, pH 7.2, temperature not specified in the publication | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(15Z)-12-oxophyto-10,15-dienoate + NADPH + H+ | - |
Arabidopsis thaliana | 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
1,4-benzoquinone + NADPH + H+ | - |
Arabidopsis thaliana | ? + NADP+ | - |
? | |
2-methylcyclopent-2-en-1-one + NADPH + H+ | - |
Arabidopsis thaliana | (2S)-2-methylcyclopentanone + NAD+ | - |
? | |
maleic acid + NADPH + H+ | - |
Arabidopsis thaliana | ? + NADP+ | - |
? | |
methyl vinyl ketone + NADPH + H+ | - |
Arabidopsis thaliana | hexanal + NADP+ | - |
? | |
N-ethyl-maleimide + NADPH + H+ | - |
Arabidopsis thaliana | ? + NAD+ | - |
? | |
trans-hex-2-enal + NADPH + H+ | - |
Arabidopsis thaliana | hexanal + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE, x * 42690, calculated from sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
12-oxophytodienoate reductase 3 | - |
Arabidopsis thaliana |
OPR3 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | absorption maxima at 280, 374 and 467 nm | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | OPR3 is a bifunctional enzyme with NADPH-dependent alpha,beta-ketoalkene double-bond reductase and monodehydroascorbate reductase activities. OPR3 mutants show a slightly less-reduced ascorbate pool in leaves in line with the MDHAR activity of OPR3 in vitro. OPR3 catalyzes the reduction of a wide spectrum of electrophilic species that share a reactivity toward the glutathione and ascorbate. 12-oxophytodienoic acid reacts with ascorbate to form an ascorbate-12-oxophytodienoic acid adduct, but in addition OPR3 has the ability to regenerate ascorbate from monodehydroascorbate | Arabidopsis thaliana |