Cloned (Comment) | Organism |
---|---|
cloning, and DNA sequence determination and analysis, expression of gene At2g06050, encoding isozyme OPR3, in Escherichia coli as N-terminally fusion protein fused to S-tag-His6-maltose binding protein | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
10 mg/ml purified recombinant isozyme OPR3 in 100 mM MES, pH 6.0, 100 mM NaCl, 0.3 mM TCEP, hanging drop vapour diffusion method, 0.002 ml protein solution with equal volume of well solution containing 8.75-10.0% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.275-0.35 M glycine, crystals are harvested into a cryoprotectant solution containing 12% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.4 M glycine, and 25% PEG 400, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH | Arabidopsis thaliana | product is an intermediate in the jasmonate biosynthesis | (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isozyme OPR3 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme OPR3 from Escherichia coli by nickel-imino-diacetic acid affinity chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH | - |
Arabidopsis thaliana | (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH | product is an intermediate in the jasmonate biosynthesis | Arabidopsis thaliana | (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OPR | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |