Cloned (Comment) | Organism |
---|---|
expression of His-tagged isozyme OPR1 in Escherichia coli, transient expression of isozyme OPR1 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study | Solanum lycopersicum |
expression of His-tagged isozyme OPR2 in Escherichia coli | Solanum lycopersicum |
expression of His-tagged isozyme OPR3 in Escherichia coli, transient expression of isozyme OPR3 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study | Solanum lycopersicum |
expression of the 3 isozymes OPR1-3 as N-terminally GST- or His-tagged enzymes in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of isozyme OPR3 mutant plants for subcellular localization study of the isozyme, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isozyme OPR1 | Solanum lycopersicum | 5829 | - |
cytosol | isozyme OPR2 | Solanum lycopersicum | 5829 | - |
cytosol | isozymes OPR1 and OPR2 | Arabidopsis thaliana | 5829 | - |
peroxisome | isozyme OPR3 | Arabidopsis thaliana | 5777 | - |
peroxisome | isozyme OPR3 | Solanum lycopersicum | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana | |
Mg2+ | - |
Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(15Z)-12-oxophyto-10,15-dienoate + NADPH | Arabidopsis thaliana | - |
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(15Z)-12-oxophyto-10,15-dienoate + NADPH | Solanum lycopersicum | - |
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH | Solanum lycopersicum | - |
(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH | Solanum lycopersicum | product is the direct precursor in the jasmonate biosynthesis | (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
additional information | Solanum lycopersicum | phylogenetic analysis | ? | - |
? | |
additional information | Solanum lycopersicum | phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response | ? | - |
? | |
additional information | Arabidopsis thaliana | phylogenetic analysis, isozyme OPR3 is induced in self-defense signalling, enzyme is involved in octadecanoid and jasmonate biosynthesis with differential roles for in the local versus the systemic wound response, cDNA micro array analysis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Col-0, 3 isozymes OPR1-3 | - |
Solanum lycopersicum | Q9FEW9 | isozyme OPR3; 3 isozymes OPR1-3 | - |
Solanum lycopersicum | Q9FEX0 | isozyme OPR2, 12-oxophytodienoate-like protein; 3 isozymes OPR1-3, 12-oxophytodienoate-like protein | - |
Solanum lycopersicum | Q9XG54 | isozyme OPR1; 3 isozymes OPR1-3 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme OPR1 from Escherichia coli by nickel affinity chromatography | Solanum lycopersicum |
recombinant His-tagged isozyme OPR2 from Escherichia coli by nickel affinity chromatography | Solanum lycopersicum |
recombinant His-tagged isozyme OPR3 from Escherichia coli by nickel affinity chromatography | Solanum lycopersicum |
recombinant tagged isozymes OPR1-3 from Escherichia coli by affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | isozyme OPR3, up-regulation in wounded leaves, low activity in unwounded, systemic leaves | Arabidopsis thaliana | - |
mesophyll | - |
Arabidopsis thaliana | - |
shoot | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(15Z)-12-oxophyto-10,15-dienoate + NADPH | - |
Arabidopsis thaliana | 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(15Z)-12-oxophyto-10,15-dienoate + NADPH | - |
Solanum lycopersicum | 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(15Z)-12-oxophyto-10,15-dienoate + NADPH | racemic mixture as substrate is accepted by isozyme OPR1 | Arabidopsis thaliana | 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH | - |
Solanum lycopersicum | (9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH | isozyme OPR1, no activity with the (9S,13S)-(15Z)-12-oxophyto-10,15-dienoate enantiomer | Solanum lycopersicum | (9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH | isozymes OPR3 | Solanum lycopersicum | (9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH | product is the direct precursor in the jasmonate biosynthesis | Solanum lycopersicum | (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH | isozyme OPR3 | Solanum lycopersicum | (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
additional information | phylogenetic analysis | Solanum lycopersicum | ? | - |
? | |
additional information | phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response | Solanum lycopersicum | ? | - |
? | |
additional information | phylogenetic analysis, isozyme OPR3 is induced in self-defense signalling, enzyme is involved in octadecanoid and jasmonate biosynthesis with differential roles for in the local versus the systemic wound response, cDNA micro array analysis | Arabidopsis thaliana | ? | - |
? | |
additional information | no activity of isozyme OPR2 with (15Z)-12-oxophyto-10,15-dienoate stereoisomers | Solanum lycopersicum | ? | - |
? | |
additional information | stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds | Arabidopsis thaliana | ? | - |
? | |
additional information | stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds | Solanum lycopersicum | ? | - |
? | |
additional information | stereospecificity of isozymes, overview, isozyme OPR3 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds | Solanum lycopersicum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OPR | - |
Arabidopsis thaliana |
OPR | - |
Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
25 | - |
assay at | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
7.5 | - |
assay at | Solanum lycopersicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | dependent on | Arabidopsis thaliana | |
flavin | dependent on | Solanum lycopersicum | |
NADPH | - |
Arabidopsis thaliana | |
NADPH | - |
Solanum lycopersicum |