Cloned (Comment) | Organism |
---|---|
expressed in Escehrichia coli as a His-tagged fusion protein | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of a ternary complex of peroxisomal 2,4-dienoyl CoA reductases (pDCR) with hexadienoyl CoA and NADP is described. The structure of pDCR refined to 1.84 A resolution reveals the absence of the tyrosine-serine pair seen in the active site of mitochondrial DCR. Instead, aspartate hydrogen-bonded to the Calpha hydroxyl via a water molecule perturbs the water molecule for protonation of the substrate | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D137A | relative activity: 3.32% (substrate: 2,4-decadienoyl CoA), 1.27% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) | Homo sapiens |
D155A | relative activity: 36.3% (substrate: 2,4-decadienoyl CoA), 59.6% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) | Homo sapiens |
D186A | relative activity: 6.21% (substrate: 2,4-decadienoyl CoA), 1.86% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) | Homo sapiens |
D268A | relative activity: 2.75% (substrate: 2,4-decadienoyl CoA), 1.14% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) | Homo sapiens |
D86A | relative activity: 1.70% (substrate: 2,4-decadienoyl CoA), 1.76% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) | Homo sapiens |
E215A | relative activity: 28.9% (substrate: 2,4-decadienoyl CoA), 7.59% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0127 | - |
(2E,4E)-2,4-decadienoyl-CoA | pH 7.4, 20°C | Homo sapiens | |
0.0716 | - |
(2E,4E)-2,4-hexadienoyl-CoA | pH 7.4, 20°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Homo sapiens | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NUI1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,4E)-2,4-decadienoyl-CoA + NADPH | - |
Homo sapiens | ? | - |
? | |
(2E,4E)-2,4-hexadienoyl-CoA + NADPH | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | analytical ultracentrifugation analysis of purified pDCR reveals that the protein exists as a mixture of monomers, dimers, and tetramers in solution | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
pDCR | - |
Homo sapiens |
peroxisomal 2,4-dienoyl CoA reductase | - |
Homo sapiens |