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Literature summary for 1.3.1.34 extracted from

  • Hua, T.; Wu, D.; Ding, W.; Wang, J.; Shaw, N.; Liu, Z.J.
    Studies of human 2,4-dienoyl CoA reductase shed new light on peroxisomal betta-oxidation of unsaturated fatty acids (2012), J. Biol. Chem., 287, 28956-28965.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escehrichia coli as a His-tagged fusion protein Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of a ternary complex of peroxisomal 2,4-dienoyl CoA reductases (pDCR) with hexadienoyl CoA and NADP is described. The structure of pDCR refined to 1.84 A resolution reveals the absence of the tyrosine-serine pair seen in the active site of mitochondrial DCR. Instead, aspartate hydrogen-bonded to the Calpha hydroxyl via a water molecule perturbs the water molecule for protonation of the substrate Homo sapiens

Protein Variants

Protein Variants Comment Organism
D137A relative activity: 3.32% (substrate: 2,4-decadienoyl CoA), 1.27% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) Homo sapiens
D155A relative activity: 36.3% (substrate: 2,4-decadienoyl CoA), 59.6% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) Homo sapiens
D186A relative activity: 6.21% (substrate: 2,4-decadienoyl CoA), 1.86% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) Homo sapiens
D268A relative activity: 2.75% (substrate: 2,4-decadienoyl CoA), 1.14% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) Homo sapiens
D86A relative activity: 1.70% (substrate: 2,4-decadienoyl CoA), 1.76% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) Homo sapiens
E215A relative activity: 28.9% (substrate: 2,4-decadienoyl CoA), 7.59% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0127
-
(2E,4E)-2,4-decadienoyl-CoA pH 7.4, 20°C Homo sapiens
0.0716
-
(2E,4E)-2,4-hexadienoyl-CoA pH 7.4, 20°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Homo sapiens 5777
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NUI1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,4E)-2,4-decadienoyl-CoA + NADPH
-
Homo sapiens ?
-
?
(2E,4E)-2,4-hexadienoyl-CoA + NADPH
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More analytical ultracentrifugation analysis of purified pDCR reveals that the protein exists as a mixture of monomers, dimers, and tetramers in solution Homo sapiens

Synonyms

Synonyms Comment Organism
pDCR
-
Homo sapiens
peroxisomal 2,4-dienoyl CoA reductase
-
Homo sapiens