Cloned (Comment) | Organism |
---|---|
into the vector pQE30 | Hordeum vulgare |
Protein Variants | Comment | Organism |
---|---|---|
C195A | mutant, constructed for the identification of the protochlorophyllide binding site | Hordeum vulgare |
C222A | mutant, constructed for the identification of the protochlorophyllide binding site | Hordeum vulgare |
C33A | mutant, constructed for the identification of the protochlorophyllide binding site | Hordeum vulgare |
C85A | mutant, constructed for the identification of the protochlorophyllide binding site | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
determined by SDS-PAGE | Hordeum vulgare |
42000 | - |
determined by SDS-PAGE | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protochlorophyllide + NADPH + H+ | Hordeum vulgare | - |
chlorophyllide + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-NTA agarose chromatography | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protochlorophyllide + NADPH + H+ | - |
Hordeum vulgare | chlorophyllide + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | oligomeric PORA-PORB complex | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
NADPH:protochlorophyllide oxidoreductase A | - |
Hordeum vulgare |
NADPH:protochlorophyllide oxidoreductase B | - |
Hordeum vulgare |
PORA | - |
Hordeum vulgare |
PORB | - |
Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Hordeum vulgare |
Organism | Comment | Expression |
---|---|---|
Hordeum vulgare | PORA represents the negatively light-regulated POR enzyme | down |