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Literature summary for 1.3.1.32 extracted from

  • Hatta, T.; Fujii, E.; Takizawa, N.
    Analysis of two gene clusters involved in 2,4,6-trichlorophenol degradation by Ralstonia pickettii DTP0602 (2012), Biosci. Biotechnol. Biochem., 76, 892-899.
    View publication on PubMed
Search for more literature for 1.3.1.32

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli Ralstonia pickettii

Organism

Organism UniProt Comment Textmining
Ralstonia pickettii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified 433fold using DEAR-Toyopearl column chromatography, phenyl-sepharose column chromatography and Mono Q column chromatography Ralstonia pickettii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-maleylacetate + NADPH
-
 Ralstonia pickettii 3-oxoadipate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
hadD
-
 Ralstonia pickettii
maleylacetate reductase
-
 Ralstonia pickettii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Ralstonia pickettii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Ralstonia pickettii

General Information

General Information Comment Organism
malfunction cells bearing a disrupted hadD gene do not grow on plates with 2,4,6-TCP Ralstonia pickettii
physiological function maleylacetate reductase is responsible for 2,4,6-trichlorophenol degradation in Ralstonia pickettii DTP0602 Ralstonia pickettii