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Literature summary for 1.3.1.32 extracted from
- Cloning, overexpression, purification, and characterization of the maleylacetate reductase from Sphingobium chlorophenolicum strain ATCC 53874 (2009), Curr. Microbiol., 58, 599-603.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | Sphingobium chlorophenolicum has assembled new catabolic pathways to degrade pentachlorophenol and use the ring-cleavage products as their carbon sources | Sphingobium chlorophenolicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21-AI cells | Sphingobium chlorophenolicum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
maleylacetate | at pH 7.0 | Sphingobium chlorophenolicum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
recombinant enzyme, determined by SDS-PAGE | Sphingobium chlorophenolicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chloromaleylacetate + NADH | Sphingobium chlorophenolicum | final enzyme in the pentachlorophenol biodegradation pathway which catalyzes the reductive dehalogenation of 2-chloromaleylacetate to maleylacetate and the subsequent reduction of malyelacetate to 3-oxoadipate | maleylacetate + NAD+ + Cl- | - |
? | |
| maleylacetate + NADH | Sphingobium chlorophenolicum | - |
3-oxoadipate + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingobium chlorophenolicum | Q8KN40 | strain ATCC 53874 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant poly-His tagged PcpE is purified to higher than 95% purity using affinity chromatography with a Ni-NTA agarose column | Sphingobium chlorophenolicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chloromaleylacetate + NADH | final enzyme in the pentachlorophenol biodegradation pathway which catalyzes the reductive dehalogenation of 2-chloromaleylacetate to maleylacetate and the subsequent reduction of malyelacetate to 3-oxoadipate | Sphingobium chlorophenolicum | maleylacetate + NAD+ + Cl- | - |
? | |
| maleylacetate + NADH | - |
Sphingobium chlorophenolicum | 3-oxoadipate + NAD+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| maleylacetate reductase | - |
Sphingobium chlorophenolicum |
| PcpE | - |
Sphingobium chlorophenolicum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
maleylacetate | at pH 7.0 in 20 mM Tris-HCl | Sphingobium chlorophenolicum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
in Tris-HCl | Sphingobium chlorophenolicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
PcpE loses approximately 60% of its maximal activity | Sphingobium chlorophenolicum |
| 8 | - |
PcpE loses approximately 60% of its maximal activity | Sphingobium chlorophenolicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Sphingobium chlorophenolicum | |
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