Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | total inhibition, reversed by thiol | Cutaneotrichosporon cutaneum | |
maleylacetate | above 300 mM | Cutaneotrichosporon cutaneum | |
p-hydroxymercuribenzoate | total inhibition, reversed by thiol | Cutaneotrichosporon cutaneum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Cutaneotrichosporon cutaneum |
81000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-maleylacetate + NADPH | Pseudomonas putida | - |
3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | Cutaneotrichosporon cutaneum | - |
3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | Sporotrichum pulverulentum | - |
3-oxoadipate + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cutaneotrichosporon cutaneum | - |
phenol-utilizing yeast | - |
Pseudomonas putida | - |
- |
- |
Sporotrichum pulverulentum | - |
lignin-degrading white-rot fungus | - |
Purification (Comment) | Organism |
---|---|
- |
Cutaneotrichosporon cutaneum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34.2 | - |
- |
Cutaneotrichosporon cutaneum |
Storage Stability | Organism |
---|---|
-25°C, 50 mM Tris/H2SO4 buffer, pH 7.6, can be stored for several months without loss of activity | Cutaneotrichosporon cutaneum |
5°C, 50 mM Tris/H2SO4 buffer, pH 7.6, can be stored for several days without loss of activity | Cutaneotrichosporon cutaneum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-maleylacetate + NAD(P)H | - |
Pseudomonas putida | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NAD(P)H | - |
Cutaneotrichosporon cutaneum | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NAD(P)H | - |
Sporotrichum pulverulentum | 3-oxoadipate + NAD(P)+ | - |
ir | |
2-maleylacetate + NADPH | - |
Pseudomonas putida | 3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | - |
Cutaneotrichosporon cutaneum | 3-oxoadipate + NADP+ | - |
ir | |
2-maleylacetate + NADPH | - |
Sporotrichum pulverulentum | 3-oxoadipate + NADP+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Cutaneotrichosporon cutaneum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.8 | - |
Cutaneotrichosporon cutaneum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas putida | |
NADH | - |
Cutaneotrichosporon cutaneum | |
NADPH | - |
Cutaneotrichosporon cutaneum | |
NADPH | - |
Sporotrichum pulverulentum |