Literature summary for 1.3.1.3 extracted from

Faucher, F.; Cantin, L.; Luu-The, V.; Labrie, F.; Breton, R.
The crystal structure of human DELTA4-3-ketosteroid 5beta-reductase defines the functional role of the residues of the catalytic tetrad in the steroid double bond reduction mechanism (2008), Biochemistry, 47, 8261-8270.

Search for more literature for 1.3.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method in 20% (w/v) PEG-4K, 0.1 M sodium cacodylate (pH 6.5), 0.2 M sodium citrate, 0.5 M (NH4)2SO4	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65000	-	recombinant GST-5beta-reductase fusion protein, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P51857	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione-Sepharose column chromatography and Sephacryl-S100 gel filtration	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
17,21-dihydroxy-5beta-pregnane-3,11,20-trione + NADP+ = cortisone + NADPH + H+	catalyzes the reduction of the DELTA4 double bond of DELTA4-3-ketosteroids, converting those steroids into 5beta-dihydro-3-ketosteroids	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7alpha,12beta-dihydroxy-4-cholesten-3-one + NADPH + H+	-	Homo sapiens	(5beta,7alpha,12beta)-7,12-dihydroxycholestan-3-one + NADP+	-	?
7alpha-hydroxy-4-cholesten-3-one + NADPH	-	Homo sapiens	(5beta,7alpha)-7-hydroxycholestan-3-one + NADP+	-	?
progesterone + NADPH + H+	-	Homo sapiens	pregnan-3,20-dione + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
5beta-red	-	Homo sapiens
DELTA4-3-ketosteroid 5beta-reductase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)