Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diphosphate | high concentration: F2 slight inhibition, F1 stimulation | Sus scrofa | |
NaCl | high concentration: F2 inhibition, F1 stimulation | Sus scrofa | |
SO42- | stimulates isoform F2 | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Barbital | inhibits isoform F2 | Sus scrofa | |
cyclopentane-1,1-diacetic acid NaCl | inhibits only isoform F2 | Sus scrofa | |
diphosphate | high concentration: F2 slight inhibition, F1 stimulation | Sus scrofa | |
Hexestrol | i.e. 4,4'-(1,2-diethyl-1,2-ethanediyl)bisphenol, inhibits isoform F2 | Sus scrofa | |
indomethacin | inhibits both isoforms | Sus scrofa | |
NaCl | high concentration: F2 inhibition, F1 stimulation | Sus scrofa | |
p-chloromercuribenzene sulfonate | stronger effect on isoform F2 | Sus scrofa | |
p-chloromercuribenzoate | stronger effect on isoform F1 | Sus scrofa | |
quercitrin | inhibits isoform F2 and slightly isoform F1 | Sus scrofa | |
SO42- | inhibits only isoform F1 | Sus scrofa | |
sorbinil | inhibits isoform F1 | Sus scrofa | |
Thiol-blocking reagents | F1 | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000, F2, SDS-PAGE | Sus scrofa |
39000 | - |
2 * 39000, F1, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
naphthalene dihydrodiol + NAD(P)+ | Sus scrofa | - |
? | - |
? | |
trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+ | Sus scrofa | - |
catechol + NAD(P)H | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
at least 2 proteins: monomeric form F2 and dimeric form F1 with both dihydrodiol dehydrogenase EC 1.3.1.20 and aldehyde reductase activity EC 1.1.1.2, F1 shows higher dihydrodiol activity, F2 shows higher aldehyde reductase activity | - |
Purification (Comment) | Organism |
---|---|
at least 2 proteins: monomeric form F2 and dimeric form F1 with both dihydrodiol dehydrogenase EC 1.3.1.20 and aldehyde reductase activity EC 1.1.1.2 | Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | probably identical with aldehyde reductase EC 1.1.1.2 | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | low activity | Sus scrofa | - |
brain | low activity | Sus scrofa | - |
lens | - |
Sus scrofa | - |
lung | low activity | Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-glyceraldehyde + NADPH | probable identity with aldehyde reductase EC 1.1.1.2 | Sus scrofa | ? | - |
? | |
additional information | at least 2 proteins, monomeric form F2 and dimeric form F1, with both dihydrodiol dehydrogenase EC 1.3.1.20 and aldehyde reductase activity EC 1.1.1.2, F1: specifically oxidizes trans-hydrodiols of naphthalene and benzene strictly with NADP+ as cofactor, reduction of vicinal diketones, aromatic aldehydes and glyceraldes with NADPH as cofactor | Sus scrofa | additional information | - |
? | |
additional information | not: hydroxy steroids | Sus scrofa | additional information | - |
? | |
additional information | F2: high activity towards naphthalene dihydrodiol and some alcohols, low activity towards trans- and cis-benzene dihydrodiols and some alcohols, reduction of aldoses, vicinal diketones, aromatic aldehydes and glyceraldehydes with NADPH or NADH as cofactors | Sus scrofa | additional information | - |
? | |
naphthalene dihydrodiol + NAD(P)+ | - |
Sus scrofa | ? | - |
? | |
trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+ | - |
Sus scrofa | catechol + NAD(P)H | - |
? | |
trans-naphthalene dihydrodiol + NAD(P)+ | - |
Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000, F1, SDS-PAGE | Sus scrofa |
monomer | 1 * 35000, F2, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
F2: reduction of vicinal diketones, aromatic aldehydes and glyceraldehydes + NAD(P)H | Sus scrofa |
6 | 8 | F1: reduction of vicinal diketones, aromatic aldehydes and glyceraldehydes + NADPH | Sus scrofa |
7.6 | - |
F2: oxidation of naphthalene dihydrodiol + NAD(P)+ | Sus scrofa |
10.2 | - |
F1: oxidation of trans-dihydrodiols of naphthalene and benzene + NADP+ | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sus scrofa | |
NADH | activity only with isoform F2 | Sus scrofa | |
NADP+ | - |
Sus scrofa | |
NADPH | - |
Sus scrofa |