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Literature summary for 1.3.1.14 extracted from

  • Andersen, P.S.; Jansen, P.J.G.; Hammer, K.
    Two different dihydroorotate dehydrogenases in Lactococcus lactis (1994), J. Bacteriol., 176, 3975-3982.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain MC1061 Lactococcus lactis
two different genes Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34120
-
 deduced from sequence of cDNA Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis A2RJT9 subspecies Lactococcus lactis cremoris, strain MG1363
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
 native wild type enzyme, using NAD+ as acceptor, at 30°C Lactococcus lactis
0.05
-
 recombinant wild type enzyme, using NAD+ as acceptor, at 37°C Lactococcus lactis
0.06
-
 native wild type enzyme, using 2,6-dichlorophenolindophenol as acceptor, at 30°C Lactococcus lactis
5.9
-
 recombinant wild type enzyme, using 2,6-dichlorophenolindophenol as acceptor, at 37°C Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + 2,6-dichlorophenolindophenol
-
 Lactococcus lactis orotate + reduced 2,6-dichlorophenolindophenol
-
 ?
(S)-dihydroorotate + NAD+
-
 Lactococcus lactis orotate + NADH + H+
-
 ?
dihydroorotate + acceptor acceptor: NAD+ for pyrDa gene product, fumarate for pyrDb gene product Lactococcus lactis orotate + reduced acceptor
-
 ?
additional information O2, fumarate, and NADP+ do not serve as electron acceptors Lactococcus lactis ?
-
 ?

Synonyms

Synonyms Comment Organism
DHOdehase A
-
 Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Lactococcus lactis