Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Ignicoccus hospitalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by L-Tyr | Ignicoccus hospitalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.319 | - |
prephenate | at pH 8.0 and 80°C | Ignicoccus hospitalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40200 | - |
- |
Ignicoccus hospitalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | Ignicoccus hospitalis | - |
4-hydroxyphenylpyruvate + CO2 + NADH | - |
? | |
prephenate + NADP+ | Ignicoccus hospitalis | dual cofactor specificity with a preference for NADP+ | 4-hydroxyphenylpyruvate + CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ignicoccus hospitalis | A8AAX2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Ignicoccus hospitalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | - |
Ignicoccus hospitalis | 4-hydroxyphenylpyruvate + CO2 + NADH | - |
? | |
prephenate + NADP+ | dual cofactor specificity with a preference for NADP+ | Ignicoccus hospitalis | 4-hydroxyphenylpyruvate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40612, calculated from amino acid sequence | Ignicoccus hospitalis |
Synonyms | Comment | Organism |
---|---|---|
CM-PD | bifunctional enzyme with chorismate mutase and prephenate dehydrogenase activities | Ignicoccus hospitalis |
tyrA | - |
Ignicoccus hospitalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54 | - |
melting temperature | Ignicoccus hospitalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
prephenate | at pH 8.0 and 80°C | Ignicoccus hospitalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Ignicoccus hospitalis | |
NADP+ | dual cofactor specificity with a preference for NADP+ | Ignicoccus hospitalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47 | - |
prephenate | at pH 8.0 and 80°C | Ignicoccus hospitalis |