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Literature summary for 1.3.1.124 extracted from

  • Fillgrove, K.L.; Anderson, V.E.
    The mechanism of dienoyl-CoA reduction by 2,4-dienoyl-CoA reductase is stepwise observation of a dienolate intermediate (2001), Biochemistry, 40, 12412-12421 .
    View publication on PubMed
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Organism

Organism UniProt Comment Textmining
Rattus norvegicus
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,4E)-hexa-2,4-dienoyl-CoA + NADPH + H+
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 Rattus norvegicus (3E)-hex-3-enoyl-CoA + NADP+
-
 ?
5-phenyl-(2E,4E)-2,4-pentadienoyl-CoA + NADPH + H+
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 Rattus norvegicus 5-phenyl-(3E)-penta-3-enoyl-CoA + NADP+
-
 ?

General Information

General Information Comment Organism
physiological function dienoyl-CoA and NADPH bind to the 2,4-dienoyl-CoA reductase via a sequential kinetic mechanism with a random order of nucleotide and dienoyl-CoA addition. A proton transfer step is rate limiting for (2E,4E)-hexa-2,4-dienoyl-CoA substrate, addition of a phenyl ring to the diene in 5-phenyl-(2E,4E)-2,4-pentadienoyl-CoA results in the reversal of the rate-determining step. The chemical mechanism is stepwise where hydride transfer from NADPH occurs followed by protonation of the dienolate intermediate Rattus norvegicus