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Literature summary for 1.3.1.12 extracted from
- The purification and characterisation of chorismate mutase-prephenate dehydrogenase from Escherichia coli K12 (1971), Biochim. Biophys. Acta, 229, 795-804.
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol stabilizes | Escherichia coli |
| EDTA stabilizes | Escherichia coli |
| stability in Tris-HCl buffer higher than in phosphate buffer | Escherichia coli |
| tyrosine stabilizes | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-tyrosine | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
NAD+ | - |
Escherichia coli | |
| 0.35 | 0.37 | prephenate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | 100000 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme, gel filtration | Escherichia coli |
| 78000 | 100000 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme, gel electrophoresis | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate + NAD+ | Escherichia coli | biosynthesis of L-tyrosine | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chorismate mutase-prephenate dehydrogenase bifunctional enzyme | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.1 M Tris-HCl buffer, pH 7.5, 1 mM EDTA, 1 mM DTT, 1 mM tyrosine, 70% w/v ammonium sulfate, several weeks | Escherichia coli |
| 4°C, 0.1 M phosphate buffer pH 7.6, over-night, complete loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate + NAD+ | - |
Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
| prephenate + NAD+ | biosynthesis of L-tyrosine | Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chorismate mutase-prephenate dehydrogenase bifunctional enzyme | complex with EC 5.4.99.5 | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
- |
Escherichia coli |
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