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Literature summary for 1.3.1.111 extracted from

  • Addlesee, H.A.; Gibson, L.C.; Jensen, P.E.; Hunter, C.N.
    Cloning, sequencing and functional assignment of the chlorophyll biosynthesis gene, chlP, of Synechocystis sp. PCC 6803 (1996), FEBS Lett., 389, 126-130.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bchP, sequence comparison with gene chlP from Synechocystis sp. strain PCC 6803 Cereibacter sphaeroides
gene chfP, DNA and amino acid sequence determination and analysis, the enzyme partially complements a bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides, which is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, sequence comparison with gene chP from Rhodobacter sphaeroides strain NCIB 8253 Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue encoded by gene chlP, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, phenotype, overview Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44869
-
x * 44869, sequence calculation Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bacteriochlorophyll a + 3 NADP+ Cereibacter sphaeroides
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
?
bacteriochlorophyll a + 3 NADP+ Synechocystis sp.
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
?
bacteriochlorophyll a + 3 NADP+ Cereibacter sphaeroides NCIB 8253
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides Q9Z5D4 gene bchP
-
Cereibacter sphaeroides NCIB 8253 Q9Z5D4 gene bchP
-
Synechocystis sp. Q55087 gene chlP; gene chlP
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacteriochlorophyll a + 3 NADP+
-
Cereibacter sphaeroides geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
?
bacteriochlorophyll a + 3 NADP+
-
Synechocystis sp. geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
?
bacteriochlorophyll a + 3 NADP+
-
Cereibacter sphaeroides NCIB 8253 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
?

Subunits

Subunits Comment Organism
? x * 44869, sequence calculation Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Cereibacter sphaeroides
NADP+
-
Synechocystis sp.

General Information

General Information Comment Organism
malfunction the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex Cereibacter sphaeroides
metabolism the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis Synechocystis sp.
physiological function the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis Synechocystis sp.