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Literature summary for 1.3.1.111 extracted from

  • Addlesee, H.A.; Hunter, C.N.
    Physical mapping and functional assignment of the geranylgeranyl-bacteriochlorophyll reductase gene, bchP, of Rhodobacter sphaeroides (1999), J. Bacteriol., 181, 7248-7255.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cereibacter sphaeroides
gene bchP, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS. The recombinant enzyme complements the bchP insertion mutant T6G5 Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
additional information a transposon mutant of gene bchP encoding geranylgeranyl-bacteriochlorophyll reductase possesses a structurally modified photosystem assembled with bacteriochlorophyll esterified with geranylgeraniol, rather than with phytol Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bacteriochlorophyll a + 3 NADP+ Cereibacter sphaeroides
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+ Cereibacter sphaeroides the enzyme is involved in phythylation of bacteriochlorophyll a phytyl-bacteriochlorophyll a + 3 NADP+
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides Q9Z5D4
-
-
Cereibacter sphaeroides Q9Z5D4 gene bchP
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacteriochlorophyll a + 3 NADP+
-
Cereibacter sphaeroides geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
?
bacteriochlorophyll a + 3 NADP+ activity assay is performed in the dark Cereibacter sphaeroides geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
-
Cereibacter sphaeroides phytyl-bacteriochlorophyll a + 3 NADP+
-
?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+ the enzyme is involved in phythylation of bacteriochlorophyll a Cereibacter sphaeroides phytyl-bacteriochlorophyll a + 3 NADP+
-
?

Synonyms

Synonyms Comment Organism
BchP
-
Cereibacter sphaeroides
geranylgeranyl-bacteriochlorophyll reductase
-
Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Cereibacter sphaeroides
NADPH
-
Cereibacter sphaeroides

General Information

General Information Comment Organism
malfunction a transposon mutant of gene bchP encoding geranylgeranyl-bacteriochlorophyll reductase possesses a structurally modified photosystem assembled with bacteriochlorophyll esterified with geranylgeraniol, rather than with phytol Cereibacter sphaeroides
metabolism the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis, pathway for the phytylation of bacteriochlorophyll a, overview Cereibacter sphaeroides
physiological function the bacteriochlorophyll of the purple photosynthetic bacterium Rhodobacter sphaeroides is esterified with phytol. The presence of this alcohol moiety is essential for the correct assembly of the photosynthetic apparatus. The enzyme is essential and responsible for the reduction of the alcohol moiety of (bacterio)chlorophyll to phytol Cereibacter sphaeroides