Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli K-12 | Fragaria vesca |
Crystallization (Comment) | Organism |
---|---|
crystallisation in complex with NADPH and 4-hydroxy-2,5-dimethyl-3(2H)-furanone or in complex with NADPH and 4-hydroxy-5-methyl-3(2H)-furanone. The crystal structure of FaEO was solved by molecular replacement using a published X-ray structure of a quinone oxidoreductase from Thermus thermophilus. The structure belongs to the zinc-independent medium chain dehydrogenase/reductase family | Fragaria vesca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.325 | - |
NADPH | 0.1 M K2HPO4/KH2PO4, pH 5.0 or 7.0, 30°C | Fragaria vesca | |
0.361 | - |
NADH | 0.1 M K2HPO4/KH2PO4, pH 5.0 or 7.0, 30°C | Fragaria vesca |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Fragaria vesca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fragaria vesca | O23939 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, anion exchange chromatography | Fragaria vesca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-ethylidene-4-hydroxy-5-methyl-3(2H)-furanone + NADPH + H+ | - |
Fragaria vesca | 2-ethyl-4-hydroxy-5-methyl-3(2H)-furanone + 5-ethyl-4-hydroxy-2-methyl-3(2H)-furanone + NADP+ | - |
? | |
4-hydroxy-5-methyl-2-methylene-3(2H)-furanone + NADH + H+ | kcat/Km for NADH is 7.5fold lower than for NADPH, Re-specific, i.e. the 4R-hydride of NAD(P)H is transferred | Fragaria vesca | (2R)-4-hydroxy-2,5-dimethyl-3(2H)-furanone + NAD+ | - |
? | |
4-hydroxy-5-methyl-2-methylene-3(2H)-furanone + NADPH + H+ | kcat/Km for NADH is 7.5fold lower than for NADPH, Re-specific, i.e. the 4R-hydride of NAD(P)H is transferred | Fragaria vesca | (2R)-4-hydroxy-2,5-dimethyl-3(2H)-furanone + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FaEO | - |
Fragaria vesca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | kcat/Km for NADH is 7.5fold lower than for NADPH, Re-specific, i.e. the 4R-hydride of NAD(P)H is transferred | Fragaria vesca | |
NADPH | preferred cofactor, Re-specific, i.e. the 4R-hydride of NAD(P)H is transferred | Fragaria vesca |
General Information | Comment | Organism |
---|---|---|
metabolism | (2R)-4-hydroxy-2,5-dimethyl-3(2H)-furanone is synthesized during the fruit-ripening process as a major aroma compound (55 mg/kg) of strawberry fruits | Fragaria vesca |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
NADH | 0.1 M K2HPO4/KH2PO4, pH 5.0 or 7.0, 30°C | Fragaria vesca | |
150 | - |
NADPH | 0.1 M K2HPO4/KH2PO4, pH 5.0 or 7.0, 30°C | Fragaria vesca |