Cloned (Comment) | Organism |
---|---|
gene cloned into pET21b (Novagen), without a stop codon, to incorporate a C-terminal His6-tag and transformed Escherichia coli strain BL21(DE3)pLysS (Stratagene) | Nicotiana tabacum |
Crystallization (Comment) | Organism |
---|---|
determination of the X-ray crystal structures of holo-, binary (NADP(H)-bound), and ternary (NADP+ and 4-hydroxy-3-methoxycinnamaldehyde-bound) NtDBR complexes | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2E)-3-methylpent-2-enal | substrate inhibition | Nicotiana tabacum | |
1-octen-3-one | substrate inhibition | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
NADPH | app. Km value, pH 5.4 and 25°C, with 1-nitrocyclohexene | Nicotiana tabacum | |
0.057 | - |
1-nitrocyclohexene | app. Km value, pH 7.3 and 25°C | Nicotiana tabacum | |
0.4 | - |
1-nitrocyclohexene | above value, app. Km value, pH 5.4 and 25°C | Nicotiana tabacum | |
0.516 | - |
trans-cinnamaldehyde | app. Km value, pH 7.3 and 25°C | Nicotiana tabacum | |
0.837 | - |
(2E)-3-methylpent-2-enal | app. Km value, pH 7.3 and 25°C | Nicotiana tabacum | |
1 | - |
trans-cinnamaldehyde | above value, app. Km value, pH 5.4 and 25°C | Nicotiana tabacum | |
1.032 | - |
(2E)-3-methylpent-2-enal | app. Km value, pH 5.4 and 25°C | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q9SLN8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-3-methylpent-2-enal + NADPH + H+ | - |
Nicotiana tabacum | 2-methylpentanal + NADP+ | - |
? | |
1-cyclohexene-1-carboxaldehyde + NADPH + H+ | low yields | Nicotiana tabacum | cyclohexanecarbaldehyde + NADP+ | - |
? | |
1-nitrocyclohexene + NADPH + H+ | - |
Nicotiana tabacum | nitrocyclohexane + NADP+ | - |
? | |
1-octen-3-one + NADPH + H+ | - |
Nicotiana tabacum | octan-3-one + NADP+ | - |
? | |
citral + NADPH + H+ | low yields | Nicotiana tabacum | ? | - |
? | |
trans-2-nonenal + NADPH + H+ | - |
Nicotiana tabacum | nonanal + NADP+ | - |
? | |
trans-cinnamaldehyde + NADPH + H+ | - |
Nicotiana tabacum | 3-phenylpropanal + NADP+ | - |
? | |
[(1E)-1-nitroprop-1-en-2-yl]benzene + NADPH + H+ | optically pure form of compound | Nicotiana tabacum | [(2R)-1-nitropropan-2-yl]benzene + NADP+ | - |
? | |
[(1Z)-1-nitroprop-1-en-2-yl]benzene + NADPH + H+ | poor enantioselectivity | Nicotiana tabacum | [(2S)-1-nitropropan-2-yl]benzene + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | each monomer is composed of two domains, a substrate binding or catalytic domain (residues 1-135 and 304-343) and a nucleotide-binding domain (residues 136-303) | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
NtDBR | - |
Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
1-octen-3-one | app. Kcat, pH 7.3 and 25°C | Nicotiana tabacum | |
0.66 | - |
(2E)-3-methylpent-2-enal | app. Kcat, pH 7.3 and 25°C | Nicotiana tabacum | |
0.87 | - |
trans-2-nonenal | app. Kcat, pH 7.3 and 25°C | Nicotiana tabacum | |
1.1 | - |
trans-cinnamaldehyde | app. Kcat, pH 7.3 and 25°C | Nicotiana tabacum | |
1.17 | - |
1-octen-3-one | app. Kcat, pH 5.4 and 25°C | Nicotiana tabacum | |
1.3 | - |
NADPH | app. Kcat, pH 7.3 and 25°C, with 1-nitrocyclohexene | Nicotiana tabacum | |
1.46 | - |
1-nitrocyclohexene | app. Kcat, pH 7.3 and 25°C | Nicotiana tabacum | |
2.92 | - |
trans-2-nonenal | app. Kcat, pH 5.4 and 25°C | Nicotiana tabacum | |
3.5 | - |
trans-cinnamaldehyde | value above, app. Kcat, pH 5.4 and 25°C | Nicotiana tabacum | |
9.2 | - |
(2E)-3-methylpent-2-enal | app. Kcat, pH 5.4 and 25°C | Nicotiana tabacum | |
11 | - |
1-nitrocyclohexene | value above, app. Kcat, pH 5.4 and 25°C | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
with 1-nitrocyclohexene | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | more than 1600fold higher preference for NADPH compared with NADH | Nicotiana tabacum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
(2E)-3-methylpent-2-enal | app. Ki value, pH 5.4 and 25°C | Nicotiana tabacum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
trans-cinnamaldehyde | pH 7.3 and 25°C | Nicotiana tabacum | |
2.8 | - |
trans-cinnamaldehyde | pH 5.4 and 25°C | Nicotiana tabacum | |
16 | - |
1-nitrocyclohexene | pH 5.4 and 25°C | Nicotiana tabacum | |
25.6 | - |
1-nitrocyclohexene | pH 7.3 and 25°C | Nicotiana tabacum |