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Literature summary for 1.3.1.10 extracted from

  • Kim, K.H.; Park, J.K.; Ha, B.H.; Moon, J.H.; Kim, E.E.
    Crystallization and preliminary X-ray crystallographic analysis of enoyl-ACP reductase III (FabL) from Bacillus subtilis (2007), Acta Crystallogr. Sect. F, 63, 246-248.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 0.04 M MgCl2, 0.05 M sodium cacodylate pH 6.0 and 11% (v/v) 2-methyl-2,4-pentanediol at 4°C Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-chelated Hi-Trap column chromatography, Hi-Trap Blue HP column chromatography, Q-Sepharose column chromatography, and Superdex-75 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-[acyl-carrier protein] + NADP+
-
 Bacillus subtilis trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH + H+
-
 ?
acyl-[acyl-carrier protein] + NADP+
-
 Bacillus subtilis 168 trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
enoyl-ACP reductase III
-
 Bacillus subtilis
FabL
-
 Bacillus subtilis
trans-enoyl-[acyl-carrier-protein] reductase
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent Bacillus subtilis