Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | - |
Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | part of fatty acid synthesis reducing double bonds of a great varity of acyl thioesters | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
no separation from NADH specific enzyme | Escherichia coli |
sonification and chromatography using DEAE-Cellulose, Sephadex G-100, Hydroxylapatite | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.31 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 10 mM phosphate buffer, pH 6.5, 5 mM 2-mercaptoethanol, inactivation in 2-3 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hexenoyl-[acyl-carrier protein] + NADPH | - |
Escherichia coli | hexanoyl-[acyl-carrier protein] + NADP+ | - |
? | |
crotonyl-[acyl-carrier protein] + NADPH | - |
Escherichia coli | butyryl-[acyl-carrier protein] + NADP+ | - |
? | |
additional information | active on substrates with acyl chain length C4-C16 | Escherichia coli | ? | - |
? | |
additional information | inactive with enoyl-CoA substrates | Escherichia coli | ? | - |
? | |
additional information | part of fatty acid synthesis reducing double bonds of a great varity of acyl thioesters | Escherichia coli | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5 min stable | Escherichia coli |
60 | - |
5 min, inactivation | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
no measurements below pH 6 carried out | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
inactive above pH 8, no measurement below pH 6 | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
unstable at | Escherichia coli |