Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | VDE activation is triggered by a pH reduction in the thylakoids lumen occurring under saturating light, mechanism of the VDE activation, overview. VDE activation relies on a robust and redundant network, in which the four residues D98, D117, H168 and D206 play a major role | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D117A | site-directed mutagenesis, the mutant shows 60% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
D117A | the mutant shows 40% of wild type activity | Arabidopsis thaliana |
D206I | site-directed mutagenesis, the mutant shows 56% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
D206I | the mutant shows 44% of wild type activity | Arabidopsis thaliana |
D86A | site-directed mutagenesis, the mutant shows 19% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
D86A | the mutant shows 86% of wild type activity | Arabidopsis thaliana |
D98L | site-directed mutagenesis, the mutant shows 41% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
D98L | the mutant shows 59% of wild type activity | Arabidopsis thaliana |
D98L/D117A | site-directed mutagenesis, the mutant shows 59% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
D98L/D117A | the mutant shows 41% of wild type activity | Arabidopsis thaliana |
D98L/D117A/D206I | site-directed mutagenesis, the mutant shows 84% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
D98L/D117A/D206I | the mutant shows 16% of wild type activity | Arabidopsis thaliana |
D98L/D117A/D206I/H168A | the mutant shows 6% of wild type activity | Arabidopsis thaliana |
D98L/D117A/D206I/H168A | site-directed mutagenesis, the mutant shows 94% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
H168A | site-directed mutagenesis, the mutant shows 80% reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
H168A | the mutant shows 20% of wild type activity | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
thylakoid | - |
Arabidopsis thaliana | 9579 | - |
thylakoid | lumen | Arabidopsis thaliana | 9579 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
antheraxanthin + L-ascorbate | Arabidopsis thaliana | - |
zeaxanthin + L-dehydroascorbate + H2O | - |
? | |
violaxanthin + 2 L-ascorbate | Arabidopsis thaliana | - |
zeaxanthin + 2 L-dehydroascorbate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
antheraxanthin + L-ascorbate | - |
Arabidopsis thaliana | zeaxanthin + L-dehydroascorbate + H2O | - |
? | |
violaxanthin + 2 L-ascorbate | - |
Arabidopsis thaliana | zeaxanthin + 2 L-dehydroascorbate + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
VDE | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | enzyme activation is triggered by a pH reduction in the thylakoids lumen occurring under saturating light | up |
General Information | Comment | Organism |
---|---|---|
evolution | the four putative activation residues D98, D117, H168 and D206 play are all conserved in plants but not in diatoms | Arabidopsis thaliana |
additional information | molecular dynamics and simulations, overview | Arabidopsis thaliana |
physiological function | the carotenoid zeaxanthin, synthesized from violaxanthin by violaxanthin de-epoxidase plays a major role in the protection from excess illumination | Arabidopsis thaliana |