Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Origami B cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D114A | inactive | Arabidopsis thaliana |
D114N | inactive | Arabidopsis thaliana |
D177A | inactive | Arabidopsis thaliana |
D177N | inactive | Arabidopsis thaliana |
D178A | the mutant shows 56% activity compared to the wild type enzyme | Arabidopsis thaliana |
F123A | the mutant shows 34% activity compared to the wild type enzyme | Arabidopsis thaliana |
F155A | the mutant shows 5% activity compared to the wild type enzyme | Arabidopsis thaliana |
H121A | the mutant shows 5% activity compared to the wild type enzyme | Arabidopsis thaliana |
N167A | the mutant shows 121% activity compared to the wild type enzyme | Arabidopsis thaliana |
Q119A | the mutant shows 32% activity compared to the wild type enzyme | Arabidopsis thaliana |
Q153A | the mutant shows 75% activity compared to the wild type enzyme | Arabidopsis thaliana |
Q153E | the mutant shows 60% activity compared to the wild type enzyme | Arabidopsis thaliana |
Q153L | the mutant shows 30% activity compared to the wild type enzyme | Arabidopsis thaliana |
T245A | the mutant shows 82% activity compared to the wild type enzyme | Arabidopsis thaliana |
W179A | the mutant shows less than 2% activity compared to the wild type enzyme | Arabidopsis thaliana |
W179N | the mutant shows less than 2% activity compared to the wild type enzyme | Arabidopsis thaliana |
Y175F | the mutant shows 62% activity compared to the wild type enzyme | Arabidopsis thaliana |
Y198F | inactive | Arabidopsis thaliana |
Y214F | the mutant shows less than 2% activity compared to the wild type enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin A | - |
Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
thylakoid membrane | - |
Arabidopsis thaliana | 42651 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q39249 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
antheraxanthin + ascorbate | - |
Arabidopsis thaliana | zeaxanthin + dehydroascorbate + H2O | - |
? | |
violaxanthin + ascorbate | - |
Arabidopsis thaliana | antheraxanthin + dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
VDE | - |
Arabidopsis thaliana |