Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Nova-Blue cells | Forsythia x intermedia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39400 | - |
x * 39400, calculated from amino acid sequence | Forsythia x intermedia |
59000 | - |
gel filtration | Forsythia x intermedia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Forsythia x intermedia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, Affinity Blue Gel filtration, phenyl Sepharose column chromatography, hydroxyapatite column chromatography, 2,5 ADP-Sepharose column chromatography, Affinity-Yellow gel filtration, and Superose 12 gel filtration | Forsythia x intermedia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Forsythia x intermedia | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000083 | - |
crude extract, at pH 7.4 and 30°C | Forsythia x intermedia |
0.255 | - |
after 3060fold purification, at pH 7.4 and 30°C | Forsythia x intermedia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-pinoresinol + NADPH + H+ | - |
Forsythia x intermedia | (+)-lariciresinol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | x * 35000-36000, SDS-PAGE | Forsythia x intermedia |
monomer or dimer | x * 39400, calculated from amino acid sequence | Forsythia x intermedia |
Synonyms | Comment | Organism |
---|---|---|
(+)-pinoresinol/(+)-lariciresinol reductase | - |
Forsythia x intermedia |
PLR | - |
Forsythia x intermedia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Forsythia x intermedia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Forsythia x intermedia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Forsythia x intermedia |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Forsythia x intermedia | isoelectric focusing | - |
5.7 |
Forsythia x intermedia | calculated from amino acid sequence | - |
7.1 |
General Information | Comment | Organism |
---|---|---|
metabolism | (+)-pinoresinol/(+)-lariciresinol reductase is a pivotal branchpoint enzyme in lignan biosynthesis | Forsythia x intermedia |